Fig. 4

Ppa-nhr-10 mutations affect expression levels of 32 potential regulatory target genes. Differential gene expression analysis between wild-type and Ppa-nhr-10 mutant animals (tu1654 and tu1655). Bold names along the y-axis correspond to names of the mis-regulated putative targets (El_Paco_v3). Target genes that have an exact C. elegans ortholog were additionally given the public gene name of this ortholog (indicated in brackets). Panels indicate in which life history stage the putative targets genes were found. Labels within the panels indicate function information for the gene in form of WormCat terms (black) and/or predicted PFAM domains (gray). WormCat annotations were adapted from C. elegans, based on Cel-Ppa-orthogroups identified using OrthoFinder. PFAM domains were only indicated for genes we could not assign a WormCat term to, or genes whose WormCat terms ended in “unassigned”. Cutoffs used in the differential gene expression analysis with DESeq2 and for PFAM domain prediction with HMMER are indicated in the lower left. DEGs = differentially expressed genes, MMSE = minimum mean squared error, FSOS = false sign or smaller