Fig. 5

Ppa-nhr-10 mutations cause premature death during acute starvation. Comparison of overall survival curves of wild-type P. pacificus and two Ppa-nhr-10 mutants that were well-fed (A) and completely starved (B). Survival curves show the mean survival, across all replicates, over time. Transparent ribbons behind the curves indicate the associated uncertainty in form of ± 1 standard errors of the mean. Each of the two experiments (A and B) was repeated four times independently (n = 4); the total numbers of worms (n) that were tracked across all replicates are indicated on top of both plots. Indicated P-values for pairwise comparisons resulted from a log-rank test that followed a Kaplan-Meier survival probability estimation; bold and black font indicates significant differences in the pairwise comparisons of overall survival. (C) Summary of the global Kaplan-Maier statistics for both experiments (in gray boxes) next to results from Cox proportional hazard regressions (white boxes). Points indicate the estimated hazard ratios for both mutant strains within each experiment (relative to the wild-type reference level of 1.0). Intervals indicate associated uncertainty in form of 95% confidence intervals. P-values in bold black indicate significant differences between the mutant strain and the wild type