Fig. 8

Ppa-nhr-10 mutants have various dauer-related phenotypes, including impaired long-term survival. (A). Workflow of the three dauer assays. (B-D) Results of the individual phenotypic assays indicated above each plot in the workflow chart. Point ranges indicate the mean estimates across all replicates ± 1 standard error of the mean. Note that the dauers used in the exit and survival assays (C-D) were obtained from LCs without cholesterol. Additionally, all of the worms in (C) managed to exit dauer and fully resumed developments into viable adults. Hypothesis tests used a two-tailed PERMANOVA with null model residual randomization (RRPP); relative effect sizes (Z-scores) and P-values for pairwise comparisons among the three strains are reported in the upper right of each panel. Incompatibility with the null hypothesis (Z ≥ 2 and P < 0.05) indicates the presence of a strain-specific phenotype; compatibility with the null hypothesis (Z < 2 or P ≥ 0.05) supports the absence of a strain-specific phenotype. LCs = liquid cultures, rpm = rotations per minute, SDS = sodium dodecyl sulfate, SEM = standard error of the mean. All experiments were repeated multiple times independently (n = 4). Number of worms in (B): n[PS312, (-)] = 460; n[Ppa-nhr-10(tu1654), (-)] = 537; n[Ppa-nhr-10(tu1655), (-)] = 432; n[PS312, (+)] = 750; n[Ppa-nhr-10(tu1654), (+)] = 877; n[Ppa-nhr-10(tu1655), (+)] = 879. Number of worms in (C): n[PS312] = 240; n[Ppa-nhr-10(tu1654)] = 240; n[Ppa-nhr-10(tu1655)] = 240. Number of worms in (D): n[PS312] = 395; n[Ppa-nhr-10(tu1654)] = 175; n[Ppa-nhr-10(tu1655)] = 177