The more we search, the more we find: discovering and expanding the biodiversity in the ring nematode genus Xenocriconemella De Grisse and Loof, 1965 (Nematoda: Criconematidae)

Table 2 Parameters evaluating Xenocriconemella species complex delimitation based on two rRNA genes (D2-D3 expansion segments of the 28S rRNA, ITS) and one mtDNA barcoding locus, COI, for four Xenocriconemella species of the complex

Species	Gene	Intra/Inter^a	P ID (Liberal)^b	Clade Support^c	Rosenberg’s P_AB^d
Xenocriconemella macrodora	D2-D3	-	-	-	-
	ITS	-	-	-	-
	COI	0.25	0.97 (0.94, 1.0) ^e	1.00	5.9 × 10⁻³⁰
Xenocriconemella iberica sp. nov.	D2-D3	0.05	1.00 (0.97, 1.0)	1.00	4.4 × 10⁻¹⁷
	ITS	0.01	0.98 (0.88, 1.0)	1.00	1.9 × 10⁻⁴
	COI	0.12	0.99 (0.96, 1.0)	0.89	1.2 × 10⁻⁴⁴
Xenocriconemella paraiberica sp. nov.	D2-D3	0.03	1.00 (0.97, 1.0)	1.00	3.9 × 10⁻³⁵
	ITS	0.02	1.00 (0.96, 1.0)	1.00	3.2 × 10⁻⁸
	COI	0.11	0.99 (0.96, 1.0)	1.00	5.9 × 10⁻³⁰
Xenocriconemella pradense sp. nov.	D2-D3	0.03	1.00 (0.97, 1.0)	1.00	0.01
	ITS	0.01	0.98 (0.88, 1.0)	1.00	1.9 × 10⁻⁴
	COI	0.04	1.00 (0.94, 1.0)	1.00	3.9 × 10⁻¹⁶

^aIntra-species variation relative to inter-species variation
^bThe P ID (Liberal) value represents the probability (with the 95% confidence interval) for the prediction of making a correct identification of an unknown specimen of the focal species using DNA Barcoding (closest genetic distance). P ID (Liberal) values ≥ 0.93 were considered to be delimited [38]. Numbers in bold represent significant values
^cClade support: posterior probabilities from Bayesian trees
^dRosenberg’s P_AB value is the probability that the monophyly of a group of sequences is the result of random branching
^eSignificant results are indicated in bold. (-) Not obtained or not performed because only a single sequence of D2-D3 or ITS for this species is available in NCBI

ISSN: 2056-306X