Table 1 Primers for cloning and RNAi of opsin genes, and for real-time PCR of opsin and rpl18a genes
From: Green-sensitive opsin is the photoreceptor for photic entrainment of an insect circadian clock
Gene | Forward (5′> > 3′) | Reverse (5′> > 3′) |
|---|---|---|
For cloning | ||
Opsin-UV | GAGTTAATCCACATCCCGGA | AGCTACAGCTTTGCAAGTGC |
Opsin-Blue | TGCTCGACCTGCTAATGATG | ATTGCCACAGTAGCGTAGGG |
Opsin-LW | CCGCTCTGGCACGGCCTCCTG | GTAGACGGCGTTGGCCTTGGCA |
For dsRNA synthesis | ||
Opsin-UV | GCATGCATTGCCTATGACAG | TGCCAAATGCACCAATTAGA |
Opsin-Blue | TGGTATTGGTTCTGCCATCA | ATTGCCACAGTAGCGTAGGG |
Opsin-LW | GCGTGCTGGGAGTGATCT | GCCACGTCTTGGTCAGGTAG |
For real time PCR | ||
Opsin-UV | ACATTCCCCGAGCCAGA | ACCAGTCCATTTCCCACGA |
Opsin-Blue | CTTTGCTCGACCTGCTAATGA | CACAGCCTACTTCCCAACCAA |
Opsin-LW | CGCTCCTACATCCTCGTCTACTC | CGTTCATCTTCTTGGCTTGCT |
rpl18a | GCTCCGGATTACATCGTTGC | GCCAAATGCCGAAGTTCTTG |