- Research article
- Open Access
A novel photic entrainment mechanism for the circadian clock in an insect: involvement of c-fos and cryptochromes
- Yuki Kutaragi†1,
- Atsushi Tokuoka†1,
- Yasuaki Tomiyama1,
- Motoki Nose1,
- Takayuki Watanabe2,
- Tetsuya Bando3,
- Yoshiyuki Moriyama4 and
- Kenji Tomioka1Email authorView ORCID ID profile
© The Author(s). 2018
- Received: 2 July 2018
- Accepted: 4 September 2018
- Published: 18 September 2018
Entrainment to the environmental light cycle is an essential property of the circadian clock. Although the compound eye is known to be the major photoreceptor necessary for entrainment in many insects, the molecular mechanisms of photic entrainment remain to be explored.
We found that cryptochromes (crys) and c-fos mediate photic entrainment of the circadian clock in a hemimetabolous insect, the cricket Gryllus bimaculatus. We examined the effects of RNA interference (RNAi)-mediated knockdown of the cry genes, Gb’cry1 and Gb’cry2, on photic entrainment, and light-induced resetting of the circadian locomotor rhythm. Gb’cry2 RNAi accelerated entrainment for delay shifts, while Gb’cry1/ Gb’cry2 double RNAi resulted in significant lengthening of transient cycles in both advance and delay shifts, and even in entrainment failure in some crickets. Double RNAi also strongly suppressed light induced resetting. The Gb’cry-mediated phase shift or resetting of the rhythm was preceded by light-induced Gb’c-fosB expression. We also found that Gb’c-fosB, Gb’cry2 and Gb’period (Gb’per) were likely co-expressed in some optic lobe neurons.
Based on these results, we propose a novel model for photic entrainment of the insect circadian clock, which relies on the light information perceived by the compound eye.
- Circadian clock
- Clock gene
- Photic entrainment
The circadian clock is an endogenous, highly conserved timing mechanism in animals that is used to anticipate and adapt to daily environmental changes . The oscillatory mechanism of insect clocks consists of interlinked transcriptional and translational feedback loops [2–4]. The major players in the loops are so called ‘clock genes’, including period (per), timeless (tim), Clock (Clk), and cycle (cyc). It is generally thought that the products of Clk and cyc genes heterodimerize to form a CLOCK (CLK)/CYCLE (CYC) complex, which activates transcription of per and tim in the late day to early night, and PERIOD (PER) and TIMELESS (TIM) proteins form a heterodimer that then inhibits CLK/CYC transcriptional activity later at night [2, 3]. This negative feedback is thought to produce an approximately 24 h rhythm. There is an additional loop producing rhythmic expression of either Clk or cyc . This oscillatory mechanism includes vrille (vri) and Par domain protein 1 (Pdp1) for Clk [5, 6], and ecdysone induced protein 75 (E75) and hormone receptor 3 (HR3) for cyc .
An essential property of the clock is the ability to synchronize with daily environmental cycles, with sunlight as the most important time cue. The mechanism for this synchronization, or entrainment, is best understood in Drosophila, which uses CRYPTOCHROME (dCRY or CRY1) as a photoreceptor molecule. CRY1 is a flavin-based blue light receptor, and is known to be expressed in a limited number of clock neurons , where it leads to TIM degradation in a light dependent manner [9, 10], and resets the clock [11, 12]. However, many insects possess another type of CRY, CRY2 [13, 14], which is more similar to mammalian CRYs. The role of mammalian CRYs is not completely understood. Certain lines of evidence suggest they are involved in the core oscillatory mechanism, working together with PER to repress transcriptional activity of CLK and BRAIN AND MUSCLE ARNT LIKE 1 (BMAL1, the mammalian homologue of CYC) complex [15, 16]. However, some studies have shown that mammalian CRY also plays a role as a photoreceptor, and is involved in photic entrainment of the suprachiasmatic nucleus (SCN) or peripheral clocks [17, 18]. In insects, CRY2 is believed to be involved in the core clock oscillatory mechanism , mainly based on assays using cultured cell systems. The role of CRY2 is yet to be explored in vivo.
In the present study, we investigated the role of cry1 and cry2 genes in photic entrainment of the circadian clock in the cricket, Gryllus bimaculatus. In this cricket, photic entrainment solely depends on the compound eye [19, 20], and the major circadian photoreceptor molecule in the compound eye is opsin-Long Wavelength (Gb’OpLW) [21, 22]. We have previously shown that resetting of the clock by the extension of the light phase during the early subjective night includes transcriptional regulation of clock genes, with Pdp1 as the first responder to light . However, the reset mechanism in other situations, e.g. in free-running conditions or during the night, remains unknown. We have recently shown that the cricket genome includes two cry genes, which are involved in the oscillatory machinery of its internal clock . Unlike other insects, Gb’cry2 has several transcriptional variants that form a feedback loop in a specific combination with the isoforms and Gb’cry1 . Our RNAi experiments reveal for the first time that re-entrainment to shifted light cycles was rather accelerated by reduced expression of Gb’cry2, but severely disrupted by Gb’cry1 and Gb’cry2 double knock-down. We also show that Gb’c-fosB is involved in the photic entrainment pathway. Based on these results, we propose a novel model of photic entrainment of the insect circadian clock, which furthers our understanding of the insect circadian system.
Eighth instar nymphs and adult males of the cricket, Gryllus bimaculatus, were used. They were purchased or obtained from a laboratory colony maintained under standard environmental conditions, with a lighting regimen of alternating 12 h light and 12 h darkness (LD 12:12; light: 0600–1800; Japan standard time, JST) and at a constant temperature of 25 ± 0.5 °C. They were fed laboratory chow and water.
Measurement of mRNA levels
The mRNA levels of Gb’cry1 (GenBank/EMBL/DDBJ Accession No. LC202047), Gb’cry2 (LC202053), Gb’c-fosA (fra-A, LC215243), Gb’c-fosB (fra-B, LC215244), and Gb’Pdp1 were measured by quantitative real-time polymerase chain reaction (qPCR) . Total RNA was extracted and purified from six adult male optic lobes with TRIzol Reagent (Invitrogen, Carlsbad, CA, USA), and treated with DNase I to remove contaminating genomic DNA. About 500 ng total RNA from each sample was reverse transcribed with random 6mers using PrimeScript RT reagent kit (TaKaRa, Shiga, Japan). qPCR was performed in the Mx3000P real-time PCR system (Stratagene, La Jolla, CA, USA) using FastStart Universal SYBR Green Master (Roche, Tokyo, Japan) including SYBR Green, with primers listed in Additional file 1: Table S1. We used Gb’rpl18a (GenBank/EMBL/DDBJ Accession No. DC448653) as an internal reference gene. Quantification was based on a standard curve obtained with known amounts of template DNA. The results were analyzed using the instrument vendor-associated software. The values were normalized with those of Gb’rpl18a at each time point. Results of 3–8 independent experiments were used to calculate the mean ± SEM.
Double stranded RNA (dsRNA) for Gb’cry1, Gb’cry2, Gb’c-fosA, Gb’c-fos (for targeting both Gb’c-fosA and Gb’c-fosB), Gb’opsin-long wavelength (Gb’opLW) (GenBank/EMBL/DDBJ accession No. LC004297), Gb’opsin-blue (Gb’opBlue) (LC004296), and DsRed2 derived from a coral species (Discosoma sp.), were synthesized using MEGAscript High Yield Transcription Kit (Ambion, Austin, TX, USA). For Gb’cry1, Gb’cry2, Gb’c-fos, Gb’c-fosA, Gb’opLW, and Gb’opBlue, template cDNA fragments for in vitro transcription were amplified by PCR from the cricket brain cDNA library using ExTaq DNA polymerase (TaKaRa). Primers tagged with T7 or T3 promoter sequences were used for PCR amplification (primer sequences are listed in Additional file 1: Table S1). For DsRed2 dsRNA, a DsRed2 cDNA fragment was amplified from pDsRed2-N1 (Clontech, Mountain View, CA, USA) with primers listed in Additional file 1: Table S1. Amplified fragments were purified with phenol/chloroform and precipitated with ethanol. RNA was synthesized from each of these cDNA fragments using T7 or T3 RNA polymerase. Synthesized RNA was extracted with phenol/chloroform, and suspended in 50 μl TE buffer after isopropanol precipitation. The yield and quality of RNA was assessed by spectrophotometer (Genequant Pro, Amersham Bioscience, Piscataway, NJ, USA), and equal amounts of sense and antisense RNAs were then mixed. The RNA mixture was denatured for 5 min at 100 °C and annealed by a gradual cooling to room temperature (25 °C). After ethanol precipitation, the obtained dsRNA was suspended in UltraPure DNase/RNase-Free Distilled Water (Invitrogen) and adjusted to a final concentration of 20 μM. The dsRNA solution was stored at − 80 °C until use. 760 nl of dsRNA solution was injected with Nanoliter Injector (WPI, Sarasota, FL, USA) into the abdomen of 8th instar nymphs or adult crickets anesthetized with CO2.
In situ hybridization
The adult male heads were collected at Zeitgeber time 18 (ZT18: ZT 0 and ZT 12 correspond to light-on and light-off, respectively), fixed for 24 h at 4 °C with PFA solution (4% paraformaldehyde in phosphate buffered saline), dehydrated by a series of butyl alcohol and ethanol, and embedded in paraffin. Tissues were sectioned at 6 μm and mounted on MAS-GP type A coated slides (Matsunami Glass, Osaka, Japan). In situ hybridization (ISH) was performed using ViewRNA ISH Tissue Assay (Affymetrix, Santa Clara, CA) following the manufacturer’s protocol. In brief, tissue sections were subjected to xylene deparaffinization followed by ethanol dehydration. To unmask the RNA targets, deparaffinized sections were incubated in pretreatment buffer at 90–95 °C for 10 min and digested with protease (1:100 dilution) at 40 °C for 10 min, followed by fixation with 10% neutral buffered formalin at room temperature for 5 min. Unmasked tissue sections were subsequently hybridized with the ViewRNA probe set (1:50 dilution) for 2 h at 40 °C, followed by series of post-hybridization washes. The ViewRNA probes used for detecting Gb’per (Accession No. AB375516) and Gb’cry2 were designed and synthesized by Affymetrix, covering 1027–2016 and 1548–2669 base region, respectively. A non-probe sample was utilized as a negative control. Signal amplification was achieved via a series of sequential hybridizations and washes according to the manufacture’s protocol. Signals for Gb’per and Gb’cry2 were detected with Fast Red or Fast Blue substrate, respectively. Slides were post-fixed in 10% neutral buffered formalin, mounted in Dako Ultramount mounting medium (Dako, Carpinteria, CA), observed and photographed using light microscopy (BZ-X700, KEYENCE, Osaka, Japan).
In situ RT-PCR
Heads were collected at ZT21 from adult males that were exposed to light for 1 h from ZT20. They were fixed in 4% PFA solution for 24 h at 4 °C, dehydrated by a series of butyl alcohol and ethanol, and embedded in paraffin. Tissues were sectioned at 6 μm and mounted on MAS-GP type A coated slides. The sections were pretreated with 1 U/μl DNase I (TaKaRa) in DNase buffer containing 2 U/μl RNase inhibitor (TaKaRa) at 37 °C overnight. Following the DNase I treatment, the sections were washed with RNase-free PBS and RNase-free water. One-step in situ RT-PCR was performed using RT-PCR Quick Master Mix (TOYOBO, Osaka, Japan) in a thermal cycler (Mastercycler, Eppendorf) with in situ Adapter. Final concentration of the reaction mixture was as follows: 1× RT-PCR Quick Master Mix, 2.5 mM Mn(OAc)2, 0.2 μM of forward and reverse primers for Gb’c-fosB (described in Additional file 1: Table S1), 40 U/μl RNase inhibitor (TaKaRa), 1.2 μl/100 μl of 1 mM digoxigenin (DIG)-11-dUTP (Roche) and relevant amount of RNase free water. The cDNA was synthesized at 60 °C for 30 min. PCR amplification consisted of an initial denaturation step of 94 °C for 1 min, followed by 20 reaction cycles of denaturation (94 °C, 30 s), annealing (60 °C, 30 s), and extension (72 °C, 1 min), then by termination with a final extension reaction at 72 °C for 7 min. The sections were fixed with 4% PFA solution for 10 min at 4 °C and washed in 0.1× standard saline citrate and washing buffer. After a blocking step, the sections were incubated with alkaline-phosphatase-conjugated sheep anti-digoxigenin Fab antibody (Roche). DIG-labeled PCR products were detected with 4-nitro blue tetrazolium chloride (Roche) and 5-bromo-4-chloro-3-indolyl phosphate (Roche) by incubating the sections for the relevant length of time. The sections were mounted, observed and photographed using light microscopy (BZ-X700, KEYENCE).
Locomotor activities were recorded according to Moriyama et al. . Briefly, the final instar nymphs or adult crickets were individually housed in a transparent plastic box (18 × 9 × 4.5 cm) with a rocking substratum. The number of substratum rocks was recorded every 6 min by a computerized system. Food and water were provided ad libitum. The actographs were placed in an incubator (MIR-153, Sanyo Biomedica, Osaka, Japan) with constant temperature at 25 ± 0.5 °C, and light was introduced by a cool white fluorescent lamp connected to an electric timer. The light intensity was 600–1000 lx at the animal’s level, varying with proximity to the lamp. The raw data were displayed as conventional double-plotted actograms to judge activity patterns, and statistically analyzed by the chi-square periodogram  with Actogram J (http://actogramj.neurofly.de/) . If a peak of the periodogram appeared above the 0.05 confidence level, the power value (height of the peak above the confidence level) was greater than or equal to 10, and the width of the peak was greater than or equal to 2, the period for the peak was designated as statistically significant .
The magnitude of phase shifts caused by a light pulse or a light phase extension was estimated by fitting a regression line to daily activity onsets at steady-state free-running in constant darkness (DD) after light treatments. The phase of the free-running rhythm was determined on the day of light treatment by extrapolating the regression line. The same value was obtained for the control crickets, which received the same treatments but were transferred to DD without light treatment. The magnitude of phase shift of an animal caused by light treatments was estimated by subtracting the average value of the control group from the value of each light-treated animal.
One-way analysis of variance (ANOVA) followed by a post-hoc Tukey-Kramer test was used to compare differences in the mean mRNA levels between the different time points, or in the means of magnitudes of phase shifts between groups with various treatments. To compare the means of two groups, t-test was used. In all statistical tests, the significance level was set at α = 0.05.
Gb’cry1 and Gb’cry2 play an important role in photic entrainment
Effects of Gb’cry1 and Gb’cry2 RNAi on the phase relationship between light-off and activity onset. Different lower case letters indicate that values are significantly different (ANOVA followed by Tukey-test, P < 0.05). Ψadvance and Ψdelay indicate reestablished phase relationship after 6 h phase advance or delay of LD cycles, respectively. Numbers in parenthesis indicate number of animals used
0.51 ± 0.46 h (28)
0.68 ± 0.56a h (18)
0.92 ± 0.82ab h (13)
0.60 ± 0.51 h (35)
0.69 ± 0.60a h (28)
0.73 ± 0.66ab h (21)
0.52 ± 0.78 h (15)
0.56 ± 0.74a h (16)
0.30 ± 0.28a h (14)
0.87 ± 0.84 h (45)
1.69 ± 1.60b h (14)
1.02 ± 0.76b h (18)
Effects of Gb’cry1 and Gb’cry2 RNAi on re-entrainment of the circadian locomotor rhythms in Gryllus bimaculatus. Different lower case letters indicate that values are significantly different (ANOVA followed by Tukey-test, P < 0.05)
Re-entrained animals (%)
Transient cycles (Mean ± SD; days)
6 h advance
4.0 ± 0.84a
3.57 ± 0.72a
3.27 ± 1.22a
6.8 ± 1.52b
6 h delay
4.0 ± 0.39a
3.25 ± 1.0a
1.15 ± 0.38b
5.56 ± 1.59c
We then tested the effect of Gb’cry1 and Gb’cry2 double knock-down on photic entrainment (Fig. 1d-i). For both advance and delay shifts, some fraction of the treated crickets could not synchronize within 14 days, as exemplified in Fig. 1e, h, and i. The loss of entrainment was observed in 6% (1/16) and 11% (2/18) of crickets, for advance and delay shifts, respectively. Even in the re-entrained crickets, the transient cycles were significantly greater than in control crickets, with cycles of 6.8 ± 1.52 days and 5.56 ± 1.59 days for advance and delay shifts, respectively (Table 2), and the time course and direction of re-entrainment were variable, with some crickets responding to a 6 h advance shift by synchronizing with delay shifts (Fig. 1f). This variability may be related to the wide variation of the free-running period in the double RNAi crickets . Interestingly, some crickets showed a rhythm splitting, where a component re-synchronized with a gradual advance while the other component did not respond and stayed almost at the same phase (Fig. 1e). The established final phase-relationship with LD was often abnormal in the double RNAi crickets, leading to greater Ψ values (Table 1), although the difference was significant only after advance shifts. Similar to Gb’cry2RNAi crickets, the light-induced activity during advance shifts was significantly reduced than that of the control crickets (Additional file 2: Figure S1).
Effects of Gb’cry1 RNAi and Gb’cry2 RNAi on phase responses to a light pulse
We also examined the effects of a 3 h extension of light phase (ZT12–15) on the phase of free-running locomotor rhythms in the ensuing DD. There were no significant effects of single or double dsRNA treatment of Gb’cry genes on the magnitude of the phase shifts (Fig. 2a, b).
Effects of Gb’cry1 and Gb’cry2 knock-down on photic responses of Gb’Pdp1
We next examined the effects of Gb’cry1RNAi and Gb’cry2RNAi treatment on light-induced Gb’Pdp1 upregulation, which is the first responder to light phase extension at early subjective night . We measured the mRNA levels of Gb’Pdp1 1 h after light phase extension starting at ZT12, or 1 h after light exposure starting at ZT20.
In all RNAi crickets, Gb’Pdp1 was upregulated 1 h after light-on at ZT12 in comparison to the control without light exposure, while no significant changes were observed in Gb’Pdp1 levels when a light pulse was given at ZT20 (Fig. 2c). The results were similar to those observed in untreated crickets , suggesting that Gb’crys are not upstream components of the transcription-dependent entrainment pathway.
Gb’c-fosB is involved upstream of Gb’crys
We then examined the effect of Gb’c-fosRNAi on the light-induced resetting of the circadian locomotor rhythm. Gb’c-fosA and Gb’c-fosB were both targeted because their nucleotide sequences are mostly identical, and we could not make a specific knock-down of Gb’c-fosB. The dsRNA treatment effectively knocked down the Gb’c-fosB mRNA levels to approximately 25% of that of the DsRed2RNAi controls (Additional file 3: Figure S2). The Gb’c-fosRNAi had no significant effect on the locomotor rhythm; the treated crickets showed a rhythm synchronized to LD cycles and free-running in DD (Fig. 3b). There was no significant difference in free-running period in DD between DsRed2RNAi crickets (23.70 ± 0.38 [mean ± SD] h, n = 9) and Gb’c-fosRNAi crickets (23.81 ± 0.30 h, n = 8) which were transferred directly to DD. A 3 h light pulse given at CT12 or ZT20 delayed or advanced the rhythm in Gb’c-fosRNAi by − 1.32 ± 0.86 h (n = 19) and 1.24 ± 0.59 h (n = 10), respectively, but the magnitude was significantly smaller than that of DsRed2RNAi control crickets (Fig. 3b, c). We also used dsRNA specific for Gb’c-fosA, and found no significant effects on advance shifts caused by a 3 h light pulse at ZT20 (Additional file 4: Figure S3). The suppression of phase-shifts by Gb’c-fosRNAi was therefore apparently caused by knockdown of Gb’c-fosB. However, no suppression was observed when light phase was extended by 3 h at ZT12 (Fig. 3b, c).
Following RNAi of Gb’opLW, light induced phase shifts were significantly reduced in all light treatments (Fig. 3b, c), being consistent with previous observations [21, 22]. We further examined the effects of Gb’c-fos knock-down on Gb’Pdp1 levels in response to light phase extension. In Gb’c-fosRNAi crickets, Gb’Pdp1 mRNA levels were up-regulated when the light phase was extended at ZT12, while no significant changes were observed when light was given at ZT20 or CT12 (Fig. 3d), similar to control crickets , suggesting that the Pdp1-dependent entrainment pathway is independent of the c-fos pathway.
We then examined the effects of Gb’cry knock-down on Gb’c-fosB mRNA levels. We found that Gb’cry1 or Gb’cry2 single knock-down or double knock-down did not affect light-induced upregulation of Gb’c-fosB at CT12 or ZT20 (Fig. 4c, d), suggesting that Gb’crys are downstream of Gb’c-fosB.
Gb’cry2, Gb’per, and Gb’c-fosB are expressed in the optic lobe
Gb’cry1 and Gb’cry2 play separate roles in photic entrainment
In insects, cry1 and cry2 are thought to encode a photoreceptor involved in photic entrainment and a transcriptional repressor in the clock oscillatory mechanism, respectively [14, 29–31]. However, we have recently shown that both Gb’cry1 and Gb’cry2 form an oscillatory loop that can operate independently of the Gb’per/Gb’tim loop in G. bimaculatus . In the present study, we have clearly shown that Gb’cry1 and Gb’cry2 are both involved in the photic entrainment pathway of the cricket’s circadian clock in a manner different from that of Drosophila . Double knock-down of Gb’cry1 and Gb’cry2 severely disrupted re-entrainment to shifted light cycles, with some crickets losing entrainability and others attaining longer transient cycles (Fig. 1, Table 2). The Gb’cry-dependent entrainment pathway is apparently different from the transcription-dependent entrainment pathway, or Gb’Pdp1 pathway, which includes Gb’Pdp1 as the first responder and works only when the light phase is extended . The two Gb’cry genes most likely play different roles, since the effect of double RNAi was not a simple combination of the single knock-down effect for each of these genes.
The Gb’cry2 knock-down accelerated re-synchronization of the locomotor rhythm to delayed LDs (Fig. 1c). Although we still need to examine whether this is true entrainment, this fact is reminiscent of the report that cry knock-out mice have significantly larger phase delays than wild-type mice . Because Gb’cry2 is an essential component of the cry-oscillatory loop , its knock-down results in a severe impairment of the loop. The acceleration of light resetting may be caused by the Gb’Pdp1 pathway, which is still functional even in this condition. However, entrainment through the Gb’Pdp1 pathway apparently incomplete, because double RNAi of Gb’cry genes prevents photic entrainment. This may also partly explain the large Ψ of the rhythm after phase shifts in Gb’cry double RNAi crickets (Table 1), which is similar to cry-deficient mice . In contrast, Gb’cry1RNAi only has a small effect on entrainment (Fig. 1b), because the cry-loop can be operated by Gb’cry2 variants .
The Gb’c-fosB pathway is involved in photic entrainment
Besides the Gb’c-fosB mediated pathway, Gb’crys might also be regulated by light through other mechanisms. Our previous study showed that light phase extension at early night induced Gb’cry2 upregulation with an increase of Gb’Pdp1 and Gb’Clk in nymphal crickets , suggesting the E-box mediated transcription of Gb’cry2 by Gb’Clk. Another possibility is through a D-box mediated regulation known for zebrafish clocks, in which light induces cry1a expression through a D-box mediated mechanism including PAR bZip factors, PAR and TEF-1 . Because D-boxes are found in the cis-regulatory region of Gb’cry1 and Gb’cry2, a similar mechanism might be involved in the cricket clock. These issues should be addressed in future studies.
Heterogeneous nature of clock cells
In the cricket’s clock, there must be at least two sets of clock neurons since some fraction of the Gb’cry1/Gb’cry2 double RNAi crickets showed a rhythm dissociation into two components when the light cycle was shifted by 6 h: one component still retained photic entrainability, while it was lost in the other (Fig. 1e). The heterogenous cellular organization of the clock is quite similar to that observed in Drosophila and mammals [39, 40], where entrainability to light is different between the cerebral or SCN clock neurons [41–43].
The heterogeneous cellular nature of the clock may explain the effect of Gb’cry2RNAi on the free-running locomotor rhythm. We have previously shown that Gb’cry2RNAi crickets have a wide variety of free-running periods in DD , which may reflect the underlying oscillatory neurons. The lack of Gb’CRY2 following RNAi may destroy the stable free-running of the clock neuron, hence causing dissociation among the cells. This is reminiscent of the mammalian SCN clock, where CRY plays a role as a coupling factor of clock cells that have variable free-running periods [39, 44–46].
Comparison with other insect clocks
In the present study, we found for the first time that both Gb’cry1 and Gb’cry2 play important roles in photic entrainment of the circadian clock in crickets. In hemimetabolous insects, the photoreceptor for entrainment is believed to reside in the compound eye [19, 47, 48], and this assumption has been confirmed at the molecular level in the cricket G. bimaculatus . Therefore, Gb’cry1 and Gb’cry2 are both active in the clock resetting mechanism downstream of the neurotransmission from the retinal photoreceptor. Although the exact role of Gb’cry genes should be examined in future studies, the cricket’s Gb’CRY1 likely works together with Gb’CRY2 to form an oscillatory feedback loop, which can operate independently of the Gb’per/Gb’tim loop . As for insect cry2, it is believed to be involved in the clock machinery as a clock component . However, we have shown for the first time that Gb’cry2 also plays an important role in the photic entrainment of the clock. Our results shed light on the insect clock mechanism and provide deeper understanding of its photic entrainment and diversification.
Photic entrainment is an essential property of the animal circadian clock that sets the appropriate timing of behavioral and physiological events in a 24 h cycle. Although most insects use compound eyes as photoreceptors for entrainment, the molecular mechanisms underlying entrainment remain largely unknown. Here we elucidated for the first time the molecular entrainment pathway using a hemimetabolous insect, the cricket Gryllus bimaculatus. Our results suggest that neural signals mediated by green-sensitive opsins in the compound eye first up-regulate an isoform of the bZip transcription factor gene, Gb’c-fos, which subsequently resets the clock through Gb’cry1 and Gb’cry2. These findings contribute to understanding of the photic entrainment mechanism of insect clocks.
We thank Taishi Yoshii for helpful discussions and Tsugumichi Shinohara for his assistance in manuscript preparation.
YK and KT designed the experiments. YK, AT, MN, YT, YM performed the experiments. TB and TW performed promoter analysis of Gb’cry genes and molecular analysis of Gb’c-fos gene, respectively. YK and KT analyzed the data and wrote the manuscript. All authors read and approved the final manuscript.
This study was supported in part by JSPS KAKENHI Grant Number JP15H0440017 and JP18H02480.
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