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Homology of the head sensory structures between Heterotardigrada and Eutardigrada supported in a new species of water bear (Ramazzottiidae: Ramazzottius)

Abstract

Phylum Tardigrada is represented by microscopic eight-legged panarthropods that inhabit terrestrial and marine environments. Although tardigrades are emerging model animals for areas of research including physiology, evolutionary biology, and astrobiology, knowledge of their external morphology remains insufficient. For instance, homologies between marine and terrestrial relatives largely remain unexplored. In the present study we provide detailed pictures of the head sensory organs in a new tardigrade, Ramazzottius groenlandensis sp. nov. Specimens were collected from a mixed moss and lichen sample on Ella Island, East Greenland. The new species differs from congeneric species in the presence of polygonal sculpturing on the dorsal cuticle, which is accentuated in the posterior region of the body, a lateral papilla on leg IV, and distinctive egg morphology. A Bayesian phylogenetic analysis (18S rRNA + 28S rRNA + COI) places the new species within the genus Ramazzottius with high confidence. Interestingly, the new species shows a full set of well-developed cephalic organs, which correspond to all sensory fields found in eutardigrades. Details on the full set of head organs were present only for heterotardigrades. The surface of these organs is covered with small pores, which presumably play a sensory role. This discovery suggests the homology of head sensory structures between heterotardigrades and eutardigrades, implying that the distinctive arrangement and positioning of sensory organs on the head is a plesiomorphic feature of tardigrades. Moreover, we find that the Ramazzottius oberhaeuseri morphotype forms a morphogroup, not a monophyletic species complex.

Background

Tardigrades (also known as water bears) are eight-legged microscopic metazoans that form a separate phylum included in the Panarthropoda. These animals are important members of the meiofaunal community [1] and live in a wide variety of habitats from aquatic to terrestrial environments, from deep seas to high mountains. They can be found in sediments, soil, bryophytes, lichens, and even on glaciers [2,3,4]. Thanks to their ability to enter into a latent, ametabolic life stage—cryptobiosis—some tardigrades can survive extreme conditions such as low and high temperatures, high irradiation, or pressure extremes [5,6,7]. Due to their resistance and simple body plan, some tardigrades, such as Hypsibius exemplaris Gąsiorek, Stec, Morek & Michalczyk, 2018 [8] and Ramazzottius varieornatus Bertolani & Kinchin, 1993 [9] have become renowend model organisms [10,11,12,13]. However, the evolution and links between their morphological traits, as well as their ancestral states, remain unclear (however, see Fig. 2.2 in [14]), and the homology of inter-specifically variable morphologies is underexplored.

To date, approximately 1,500 species of tardigrades have been described worldwide [15]. These are grouped into two classes: Eutardigrada and Heterotardigrada. The two groups differ in their external morphology and evolutionary history [16]. Heterotardigrades live in both terrestrial and marine ecosystems and possess several pairs of sensory organs on the head, such as cirri and clavae. For instance, echiniscoideans have ten head sensory organs, including a pair of internal/external/lateral cirri and a pair of primary/secondary clavae, while marine heterotardigrades [17] have up to 13 sensory organs, including a pair of internal/external/lateral cirri, a pair of primary/secondary/tertiary clavae, and an unpaired median cirrus [18, 19]. In contrast, sensory organs are significantly reduced or completely absent in eutardigrades. Although several neuroanatomical studies have revealed that some representatives of eutardigrades have sensory fields that may be homologous to the head sensory organs of heterotardigrades [20,21,22,23], whether these possibly homologous sensory organs appear on the surface of eutardigrades remains unresolved. Recently, a neuroanatomical comparative study involving Echiniscus testudo (Doyére, 1840) [24] and Hypsibius exemplaris [8] suggested possible homology between the cephalic sensory fields of the eutardigrades and the cephalic sensory organs of heterotardigrades [20].

Homology, defined as “the possession by two or more species of a trait derived, with or without modification, from their common ancestor” [25], is a central concept in understanding the evolution of morphological traits [26]. As an evolutionarily traceable marker of certain lineages, morphological homology forms the basis for phylogenetic reconstruction [27]. The position (anatomical location) and the structure of a character are essential criteria for detecting morphological homology [28]. However, many tardigrade species have been observed and illustrated exclusively by light microscopy, which has hindered integrative understanding of details of certain morphological features. For example, head structures, such as elliptical organs [29,30,31], frontal lobes [32, 33], and cephalic papillae [21, 32, 34] are, in a small number of eutardigrades, reminiscent of the sensory organs of heterotardigrades. However, in some eutardigrade species these structures are usually detectable only in SEM [35,36,37], and are rarely observable under the light microscopy. Observation of tardigrades under SEM could thus yield information on the detailed surface morphological features and provide insights into homologies between marine heterotardigrades and limnoterrestrial eutardigrades.

Ramazzottius Pilato & Binda, 1986 is a genus of limnoterrestrial eutardigrades occurring worldwide, including polar regions and high mountains [35]. Ramazzottius species are characterised by the presence of apophyses for the insertion of the stylet muscles (AISM) in the shape of blunt-hooks and articulated external claws. Some species have paired elliptical organs on the head [38,39,40]. Representatives of Ramazzottius are considered xerophilic, often exposed to sunlight, and can be found in substrata such as bryophytes and lichens [41]. The vast Arctic tundra in Greenland, with its abundant cryptogams, thus forms a highly suitable habitat for tardigrades. However, of the twenty-nine valid Ramazzottius species [15], only two species—R. montivagus (Dastych, 1983) [42] and R. oberhaeuseri (Doyère, 1840) [24]—have been documented from Greenland [43]. Particularly, R. oberhaeuseri, one of the earliest described tardigrade species, is the only Ramazzottius species reported from East Greenland [44]. Moreover, because R. oberhaeuseri was established with insufficient morphological and morphometric data, doubts have persisted regarding its taxonomic validity [31]. A recent integrative study even revealed that several species exist under the name R. oberhaeuseri, forming the Ramazzottius oberhaeuseri complex [31].

Here we provide an integrative description of a new species R. groenlandensis sp. nov. from Ella Island, East Greenland, with partial molecular sequences of three genes (a small ribosome subunit (18S rRNA), a large ribosome subunit (28S rRNA), and cytochrome oxidase c subunit I (COI)). Notably, SEM images of this new species show a set of head sensory organs that are most likely homologous to the sensory organs of heterotardigrades.

Materials & methods

Sample processing

During the 2019 summer season, the KOPRI (Korea Polar Research Institute) palaeontology team collected a sample of mixed bryophytes and lichens from limestone near Lake September (72°50'51.23"N, 25°5'8.71"W, 471 m above sea level (a.s.l.)) (Supplementary Fig. 1). The dry sample was kept in a plastic bag, brought to KOPRI (Incheon, Korea), and stored at 4ºC for two months. Subsequently, the sample was placed on a dish filled with Volvic® water and was squeezed over a Petri dish. Tardigrades were retrieved from the supernatant under a stereomicroscope (Leica M205C).

Microscopy and imaging

For light microscopic observation, specimens were prepared following a previously reported method [45]. Tardigrades were relaxed at 60ºC for 30 min and were mounted on a microscope slide in Hoyer’s medium. Subsequently, the slides were dried seven days at 60ºC, sealed with nail polish, and examined under a differential interference contrast (DIC) microscope (Carl Zeiss Axio Imager 2), with the camera AxioCam HRc.

For SEM observation, specimens were prepared following a previously reported method [46]. First, the tardigrades were incubated at 60ºC for 30 min. After fixation in 4% formaldehyde solution, individuals were washed three times with distilled water. Afterwards, specimens were subjected to a water/ethanol series and an ethanol/hexamethyldisilazane (HMDS) series subsequently, with 10% increasing concentration at 10-min intervals (from 10 to 100%), following a previously reported method [47]. Buccal-pharyngeal apparatuses were collected after tardigrades discarded them during molting. Dried animals and buccal-pharyngeal apparatuses were then mounted on SEM stubs using an eyebrow and coated with a thin layer of gold. SEM observations were made using a field emission SEM JSM-7200F, at KOPRI.

Morphometrics

Selection of characters for the morphometry and the morphological terminology follow those of the previous references [31, 39]. All measurements are given in micrometers (µm) and were conducted under the DIC microscope. Characters were measured when the specimens were mounted in a suitable orientation on the slide. Body length was measured from the anterior tip to the posterior end of the body, excluding the legs IV. The pt index is the percent ratio of the length of a character to the length of buccal tube [48]. For measurements of claws, the scheme described in [49] as adapted by [31] was used.

For species identification and differentiation, original descriptions and redescriptions were used [9, 31, 35, 36, 38, 50,51,52,53,54,55,56,57,58,59,60,61].

Genotyping

DNA was extracted from six individuals using QIAamp DNA Micro Kit. A PCR mixture was prepared with a total volume 25 µl, containing 12.5 µl Takara EmeraldAmp® PCR Master Mix, 2 µl of DNA template, 0.25 µl of each primer and 10 µl of ddH2O. Three DNA fragments were sequenced, namely, the small ribosome subunit (18S rRNA), the large ribosome subunit (28S rRNA) and the cytochrome oxidase c subunit I (COI). The PCR settings followed those described in a previous method [31]; primers and original references for PCR settings of all partial genes are listed in Supplementary Tables 1 & 2. The PCR products were sent to a commercial company for sequencing (Cosmogenetech, Korea). The sequences were processed in Geneious v. 9.0.5 (https://www.geneious.com) and submitted to GenBank.

Genetic distance and phylogenetics

Phylogenetic analysis was conducted using concatenated 18S rRNA + 28S rRNA + COI sequences of Ramazzottiidae that belong to fourteen taxa, with Hypsibius convergens (Urbanowicz, 1925) [62] as the outgroup. For the concatenated data set, we selected taxa for which at least two sequences among 18S, 28S, and COI were available in NCBI. We used fragments of sequences of specific species, originating from single specimens (vouchers) or specimens identified recently by using integrative approach, in order to prevent possible confusion arising from taxonomic misidentification. Sequences were downloaded from GenBank, a full list of accession numbers is given in Supplementary Data 1. 18S rRNA and 28S rRNA sequences were aligned using the Q-INS-i method, and COI sequences were aligned using G-INS-1 in MAFFT online service [63] and checked manually in BioEdit v. 7.0.5.3 [64]. The sequences were concatenated in the following order: 18S rRNA, 28S rRNA, and COI.

Partitionfinder v. 2.1.1 [65], under Bayesian Information Criterion (BIC), was used to find the best scheme of partitioning and substitution models. The following models were suggested: TRNEF+I for 18S rRNA, TRN+I for 28S rRNA, and TIM+I, TRN+G, TRNEF+G for the first, second, and third codon positions of COI, respectively. Bayesian inference (BI) posterior probabilities (PP) were calculated using MrBayes v. 3.2.6 [66]. Two random starting trees, each of four Metropolis coupled Markov chains Monte Carlo method, were launched for 3 × 107 generations. Trees were sampled every 1,000 generations and the initial 10% trees were discarded as burn-in. Convergence was assessed by checking the standard MrBayes convergence diagnostics: estimated sample size scores > 200, average standard deviation of split frequencies values < 0.01, and potential scale reduction factor values ~ 1.00. Obtained tree samples were summarized as a majority rule consensus tree. The final consensus tree was visualized using FigTree v. 1.4.4.

Additionally, we conducted another phylogenetic analysis based on COI. We included all available Ramazzottius COI sequences in the dataset (EF620418 and KU900021 were excluded due to short sequence length.). Fifty-three sequences, including R. groenlandensis sp. nov., were analyzed. The methods of alignment, model search and phylogenetic analysis are identical to those described above. The suggested model from Partitionfinder v.2.1.1 was TRN+I for the first, and TRN+G for second, and TRNEF+G for third codon positions of COI, respectively.

Pairwise distances between nucleotide sequences were calculated using a distance model for all codon positions, as implemented in MEGA X [67]. p-distance calculations for all positions containing gaps and missing data were eliminated. The analysis of COI involved 53 Ramazzottius sequences, and the analyses of 18 and 28S rRNA involved eleven and eight nucleotide sequences, respectively (including one sequence of R. groenlandensis sp. nov. with other ramazzottiids), and the final dataset had sequences with lengths of 448 (COI), 820 (18S) and 738 (28S), respectively. Using data sets for COI, we performed a genetic species delimitation analyses by Automatic Partitioning (ASAP [68]). Analyses were performed on https://bioinfo.mnhn.fr/abi/public/asap/asapweb.html with default settings. The results of these analyses are given in Supplementary Data 3.

Results

Taxonomic account

Phylum: Tardigrada Doyère, 1840 [24].

Class: Eutardigrada Richters, 1926 [69].

Order: Parachela Schuster et al., 1980 [70].

Superfamily: Hypsibioidea Pilato, 1969 [71] (in Marley et al., 2011 [39]).

Family: Ramazzottiidae Sands et al., 2008 [72].

Genus: Ramazzottius Binda and Pilato, 1986 [73].

Ramazzottius groenlandensis sp. nov.

Synonyms: Ramazzottius cf. rupeus in [37], Ramazzottius cf. oberhaeuseri species 2 [31].

urn:lsid:zoobank.org:act:2964B209-9AE6-477B-9626-9AE28342B8C0.

Examined material

Fifty-eight animals and three eggs on slides in Hoyer’s medium (2 eggs were ruptured during preparation), 181 animals and three eggs mounted on stubs for SEM observations.

Type repositories

The holotype (slide code: KOPRIF 2019-Ella-Rama 01), 52 paratype specimens (slide codes: KOPRIF 2019-Ella-Rama 02–53), three egg specimens (slide codes: KOPRIF 2019-Ella-Rama Egg 01–03), and 12 SEM stubs including 181 animal specimens and three egg specimens (stub codes: KOPRIF 2019-SEM-Ella-Rama 01–12) were deposited in the KOPRI Paleontology collection (Division of Earth Sciences, KOPRI, Korea); five paratypes (slide codes: KOPRIF 2019-Ella-Rama 54–58), were deposited at the Department of Animal Taxonomy and Ecology at Adam Mickiewicz University, Poznań, Poland.

Type locality

72°50'51.23"N, 25°5'8.71"W, 471 m a.s.l.: the limestone deposit near Lake September, Ella Island, Greenland.

Etymology

The name groenlandensis refers to the locality, Greenland, where the species was formally identified and described.

General description

Figs. 1, 2, 3, 4 and 5; measurement and basic statistics in Table 1; raw data in Supplementary Data 4.

Fig. 1
figure 1

Ramazzottius groenlandensis sp. nov. Differential interference contrast microscope (DIC) images and SEM images: A, B DIC images; C, D SEM images. A a living specimen. B the holotype. C, D dorsal view and oblique lateral view

Fig. 2
figure 2

The cuticular sculpturing of Ramazzottius groenlandensis sp. nov. Differential interference contrast microscope (DIC) images and SEM images: AC DIC images; DF SEM images. A, D cuticular surface of the head region. B, E cuticular surface of the middle part of the trunk. C, F cuticular surface of the posterior part of the trunk

Fig. 3
figure 3

Head sensory organs of Ramazzottius groenlandensis sp. nov. Differential interference contrast microscope (DIC) images and SEM images: AD SEM images; E DIC images. A head region. B frontal lobe. C centrodorsal organ (CO). D, E elliptical organ (EO). Arrows and arrow head indicate pores and EO, respectively. AVL: anteroventral lobe; CMAS: cribriform muscle attachment site; CO: centrodorsal organ; EO: elliptical organ; PBL: peribuccal lobe

Fig. 4
figure 4

The mouth opening and the buccal-pharyngeal apparatus of Ramazzottius groenlandensis sp. nov. Differential interference contrast microscope (DIC) images and SEM images: A, B, EI SEM images; C, D DIC images. A anterior view of the mouth and the peribuccal lobes. Arrow and arrowhead indicate the peribuccal lobe and the mouth opening, respectively. B oral cavity armature with two bands of teeth and perforated area. Arrow and arrowhead indicate the first band and the second band of teeth, respectively. C ventral view of the buccal-pharyngeal apparatus. D oblique lateral view of the buccal-pharyngeal apparatus. E ventral view of the buccal-pharyngeal apparatus. F lateral view of the buccal-pharyngeal apparatus. G ventral view of the apophysis for the insertion of the stylet muscles (AISM). Arrows indicate the posterior tips of AISM. H oblique lateral view of the AISM. I placoids. Arrows indicate placoid constrictions

Fig. 5
figure 5

Legs and claws of Ramazzottius groenlandensis sp. nov. Differential interference contrast microscope (DIC) images and SEM images: AD, G, I SEM images; E, F, H DIC images. A leg I B leg II CE leg IV. Arrows indicate papilla on leg IV. F claw I. G claw II. HI claw IV

Body color varied from red to brown. The pigmented surface divided by transparent transversal stripes, which disappear after mounting in Hoyer’s medium (Fig. 1A, B). Eyes absent in live animals. Dorsal cuticular sculpturing present (Fig. 1C, D), while the ventral cuticle is smooth. The anterior part of the body smooth or covered by irregular wrinkles on head (Fig. 2A, D), flat and weak polygonal sculptures in the middle region of the body (Fig. 2B, E), and strongly marked, tubercle-like structures in the caudal region of the body (Fig. 2C, F). More posteriorly, the cuticle sculpturing is larger. Under SEM, seven sensory organ-like structures present on the head (Fig. 3A): a pair of frontal lobes, a pair of anteroventral lobes (AVL), a pair of elliptical organs (EO), and a centrodorsal organ (CO). On the anterolateral sides of the head, two pairs of structures present above (frontal lobes, Fig. 3A) and below (AVL, Fig. 3A) the cribriform muscle attachment sites (CMAS, Fig. 3A), respectively. On the surface of the frontal lobes several micropores are scattered (Fig. 3B). Additionally, there is a small region which is likely to show slightly different cuticular surface on the anterodorsal part of the head (CO, Fig. 3A). This region has a pore at the centre (Fig. 3C). Two EO (Fig. 3A) with several pores present on the dorsoposterior part of the head (Fig. 3D). Only EO visible under the light microscopy (Fig. 3E).

Mouth opening anteroventral (sub-terminal). Mouth surrounded by six peribuccal lobes (Fig. 4A). Peribuccal lamellae and peribuccal papulae absent. The oral cavity possesses two bands of teeth (Fig. 4B) that are poorly visible in DIC micrographs (Fig. 4C–D). The first band consists of small triangular teeth arranged in several rows on the ring fold. The second band, composed of cone shaped-teeth in a single row, occurs behind the first band. A perforated area is present behind the second band of teeth (Fig. 4B). Buccal-pharyngeal apparatus of the Ramazzottius-type (Fig. 4C–I) [58]; i.e. asymmetrical apophysis for the insertion of the stylet muscles (AISM) with slightly longer ventral apophysis (Fig. 4D, H) and both apophyses with caudal apices. AISM has posterior tips on each lateral side (Fig. 4G) (although it is worth noting that there is a possibility that the middle tip in Fig. 4G is an artefact). The buccal tube bent ventrally after the stylet support insertion point (Fig. 4D, F). The pharyngeal bulb spherical to oval, with triangular apophyses and two macroplacoids, all clearly separated. Macroplacoids roundish; the 1st macroplacoid slightly longer than the 2nd macroplacoid. A small constriction in both 1st and 2nd macroplacoids visible (Fig. 4C, I). Microplacoid absent.

While cuticle on leg I is smooth (Fig. 5A), legs II–IV exhibit polygonal sculpturing (Fig. 5B, C). On the lateral side of leg IV, a papilla (= a gibbosity in [53]) is present (see remarks). The papilla on the leg IV varies in size (Fig. 5C–E); from a quarter to more than half of the length of the leg when the leg is fully extended (Fig. 5D). Claws of the Ramazzottius-type (Fig. 5F–I), i.e., two claws of the same leg extremely different from each other in size and shape. Primary branches of external claws and posterior claws longer than the primary branches of internal claws and anterior claws. The bases of all claws have a smooth pseudolunule. Primary branches of external/posterior claws with cuticular flexible portions, connected to the secondary branches (“oberhaeuseri group” claw in [58]). Accessory points present on all primary branches.

Table 1 Measurements (in µm) of selected morphological characters of Ramazzottius groenlandensis sp. nov. mounted in Hoyer’s medium

Eggs

Fig. 6; measurement and basic statistics in Table 2; raw data in Supplementary Data 4.

Laid free, white, spherical (Fig. 6A, B). Chorion surface between processes granulated (Fig. 6C, D). Processes show various morphology and shape (Fig. 6C–F): i.e., most processes cone-shaped whereas other processes filamentous. While most processes have bulbous tips, some have concave tips (Fig. 6F).

Fig. 6
figure 6

Eggs of Ramazzottius groenlandensis sp. nov. Differential interference contrast microscope (DIC) images and SEM images: A, D, E DIC images; B, C, F SEM images. A a whole egg. B two eggs. C granulated surface of the egg chorion. DE variable morphology of processes. F processes with a concave tip. Arrows indicate filamentous processes

Table 2 Measurements (in µm) of selected morphological characters of Ramazzottius groenlandensis sp. nov. egg. N, the number of specimens, SD, standard deviation

Morphological differential diagnosis

Ramazzottius groenlandensis sp. nov. is characterized by the presence of dorsally sculptured cuticle, several head sensory organs, two macroplacoids with constrictions and the presence of pseudolunules under the claws. The egg of Ramazzottius groenlandensis sp. nov. is characterized by processes with variable morphology and granulated chorion surface. R. groenlandensis sp. nov. differs specifically from

  • Ramazzottius affinis Bertolani, Guidetti & Rebecchi, 1994 [50] known from Monte Serra Santa, Italy (1260 m a.s.l.), found in lichen from limestone by: the presence of accessory points on external and internal primary claw branches, the lack of a thicker buccal tube wall at the stylet support insertion point (SSIP), and the pt indices of the primary branches of claw II and IV, i.e. external primary branch of claw II (78.26–80.41 in R. affinis vs. 32.3–52.3 in R. groenlandensis sp. nov.) and posterior primary branch of claw IV (78.54–85.18 in R. affinis vs. 43.5–61.0 in R. groenlandensis sp. nov.);

  • Ramazzottius bunikowskae Kaczmarek, Michalczyk & Diduszko, 2006 [38] known from lichens in Olkhon Island at Lake Baikal by: the presence of the sculpturing on legs, and the different oral cavity armature (one band in R. bunikowskae vs. two bands in R. groenlandensis sp. nov.);

  • Ramazzottius libycus Pilato, D’Urso & Lisi, 2013 [54] known from mosses in Libya by: the shape of processes (hemispherical in R. libycus vs. conical or filamentous in R. groenlandensis sp. nov.);

  • Ramazzottius littoreus Fontoura, Rubal & Veiga, 2017 [55] known from supralittoral lichens in Spain and Portugal by: the lack of the polygonal sculpturing on the head and the leg I;

  • Ramazzottius nivalis Dastych, 2006 [56] known from lichens in the Alps (3707 m a.s.l.) by: the lack of a particularly long basal flexible unit in external claws of R. groenlandensis, different pt index of the posterior primary branch of claw IV (66.6–78.6 in R. nivalis vs. 43.45–61.02 in R. groenlandensis sp. nov.) and the presence of granules on the chorion (absent in R. nivalis vs. present in R. groenlandensis sp. nov.);

  • Ramazzottius oberhaeuseri known from mosses in France by: the cuticular sculpture of the caudo-dorsal body region (smooth or weak in R. oberhaeuseri vs. robust and intense in R. groenlandensis sp. nov.) and the shape of egg processes (hemispherical in R. oberhaeuseri vs. conical or filamentous in R. groenlandensis sp. nov.);

  • Ramazzottius rupeus Biserov, 1999 [57] known from lichens in Novaya Zemlya by: sculpturing on the head (lack of sculpturing of the head in R. groenlandensis), the pt index of the posterior primary branch of claw IV (76.5 ± 3.0 in R. rupeus vs. 43.5–61.0 in R. groenlandensis sp. nov.) and the diameter of egg with processes (67.0–79.0 μm in R. rupeus vs. 92.4 μm in R. groenlandensis sp. nov.);.

  • Ramazzottius sabatiniae Guidetti, Massa, Bertolani, Rebecchi & Cesari, 2019 [58] known from Starr Nunatak, Victoria Land, Antarctica, found in mosses in soil by: egg surface (smooth in R. sabatiniae vs. granulated in R. groenlandensis sp. nov.).

Genetic comparison

A GenBank search using BLAST algorithm and our sequence data indicated that the COI sequence of Ramazzottius groenlandensis sp. nov. is most similar to that of R. cf. rupeus deposited by [37] (GenBank accession number: MG432810). 18S rRNA sequence of R. groenlandensis sp. nov. is the most similar to that of Ramazzottius varieornatus (GenBank accession number: AP013352 [6]). 28S rRNA sequence of R. groenlandensis sp. nov. is most similar to those of Ramazzottius sp. DE.002 (GenBank accession number: MG432817 [37]), and R. varieornatus (GenBank accession number: AP013352 [6]).

Length of COI partial sequence was trimmed to 658 bp (GenBank: OR596527) and the 18S rRNA sequence was trimmed to 1721 bp (GenBank: OR600266), while the 28S sequence was trimmed to 784 bp long (GenBank: OR600265). The COI sequence of Ramazzottius groenlandensis sp. nov. differs by one base pair of the COI sequence of R. cf. rupeus (Genbank accession number: MG432810) from Northern Svalbard, and by two base pairs different to that of R. cf. oberhaeuseri (Genbank accession numbers: EU251381, EU251382 [74]) from Northern Apennines, Italy.

The intraspecific and interspecific ranges of p-distances within the 53 Ramazzottius sequences (COI) are 0–3.3% and 12.5–22.8%, respectively. Interspecific ranges of p-distance within eight ramazzottiid species for 18S and six ramazzottiid species for 28S are as follows for 18S: 0.4–3.1%, and 28S: 1.6–6.7%.

ASAP

The ASAP analysis of 53 COI sequences (including all Ramazzottius COI sequences available from NCBI) identified fourteen putative species at asap score = 2.5 (Ramazzottius cf. rupeus (MG432810) [37], Ramazzottius cf. oberhaeuseri species 2 (EU251381–2) [31] are R. groenlandensis sp. nov. Other sequences are clearly different from other taxa in Ramazzottius, see Supplementary Data 3).

Phylogenetic analyses

The concatenated 18S rRNA + 28rRNA + COI phylogenetic reconstruction based on the Bayesian inference analysis shows a stable topology in the family Ramazzottiidae (Fig. 7), in which Ramazzottius groenlandensis sp. nov. is positioned as a sister group of R. varieornatus.

Fig. 7
figure 7

 A concatenated 18S rRNA + 28S rRNA + COI consensus tree based on the Bayesian Inference (BI) analysis, with Hypsibius convergens as the outgroup. Node values are given as BI posterior probability (PP) values

The COI phylogenetic reconstruction based on Bayesian inference analysis also confirms that Ramazzottius cf. rupeus (MG432810) [37] and Ramazzottius cf. oberhaeuseri species 2 (EU251381–2) in fact belong to R. groenlandensis sp. nov (Fig. 8).

Fig. 8
figure 8

 A Bayesian inference (BI) phylogenetic tree constructed using COI sequences of the genus Ramazzottius. Among the molecular species R. aff. oberhaeuseri species 1–8 identified by Stec et al. [31], R. aff. oberhaeuseri species 2 is revealed as R. groenlandensis sp nov. (as explained in the text). Node values mean BI posterior probability values. Scale bar represents substitution per position

Remarks

Several ramazzottiid species, including Ramazzottius groenlandensis sp. nov., have a papilla on the lateral part of the leg IV [31, 35, 37]. The presence of this organ has been suggested to be sexually dimorphic, being present only in males [53, 55, 75, 76]. In R. groenlandensis sp. nov., 39% of observed specimens showed a papilla on the lateral part of the leg IV. The papilla on leg IV varies in size (Fig. 5C–E); from a quarter of leg IV to more than half of the length of the leg when the leg is fully extended (Fig. 5D). It may reflect the physiological condition of each specimen, or potential deformation during the preparation process.

Due to the similar adult morphology and the lack of detailed descriptions in early reports, egg traits are used as common and the key taxonomic characters in Ramazzottius [31, 36, 38]. However, many Ramazzottius specimens have been reported without eggs, e.g., R. edmondabouti [52], R. szeptycki [59], R. semisculptus [77], R. belubellus [51], or R. thulini [54].

Additionally, a prominent intraspecific variation warns against the description of eggs based on a few specimens [78]. Particularly in the genus Ramazzottius, considerable intraspecific variation in the morphology of the egg processes has been documented from several species: e.g. R. kretschmanni Guidetti, Cesari, Giovannini, Ebel, Forschler & Schill, 2022 [53], R. littoreus, R. oberhaeuseri [31], R. sabatiniae, and R. subanomalus (Biserov, 1985) [36, 75]. Therefore, both SEM and DIC pictures and measurements of eggs should be provided in the description of species belonging to the genus Ramazzottius.

Discussion

Homology of head sensory organs

The full set of head sensory organs of Ramazzottius groenlandensis sp. nov., recognized under SEM, is most likely homologous to the sensory organs of heterotardigrades. Some eutardigrade species have one or two pairs of head sensory organs [21, 30, 32, 37, 40, 79, 80], while most eutardigrades possess specific innervated areas on the head surface, called the sensory fields—the circumoral sensory field (COS), the anterolateral sensory field (ASF), the ventrolateral sensory field (VSF), and the posterolateral sensory field (PSF) [22, 23, 81]—which are not clearly visible under DIC observation. Ramazzottius groenlandensis sp. nov. has paired sensory organs on the surface of the head, i.e., the frontal lobes, the anteroventral lobes (AVL), and the elliptical organs (EO) (Fig. 3). Based on their relative positions, the sensory organs of R. groenlandensis sp. nov. are compared to the sensory fields of other eutardigrades: frontal lobes vs. ASF; AVL vs. VSF; EO vs. PSF (Table 3). Frontal lobes and elliptical organs have been observed in several eutardigrades. Some isohypsibioid tardigrades, such as Ursulinius pappi (Iharos, 1966) [82] and Apodibius confusus Dastych, 1983 [83] (see Fig. 4B & E of [32]), possess frontal lobes. The papilla cephalica (= cephalic papilla [34]) of Halobiotus [80] is also likely to be homologous to the frontal lobes of R. groenlandensis sp. nov. Elliptical organs have been found in some hypsibioids (Calohypsibius, Cryoconicus, and Ramazzottius) [40] and isohypsibioids (Fractonotus) [30]. The position, innervation from the brain, and shape of the temporalia in Halobiotus can also be compared to the elliptical organ [21]. Apochelan tardigrades have the cephalic papillae, which can be related to AVL of R. groenlandensis sp. nov. [22]. Despite their distinct morphologies, similar innervation patterns may imply homology between the head sensory organs of heterotardigrades and the head sensory fields of eutardigrades [22, 23, 34, 84, 85]. Particularly, Gross et al. [20] suggested the homology of head sensory organs and head sensory fields between heterotardigrades and eutardigrades based on immunohistochemical data. Head sensory organs of R. groenlandensis sp. nov. possess micropores (Fig. 3), which suggests that these structures could function as mechano-chemoreceptors, as do the cirri and clavae of heterotardigrades [19].

The centrodorsal organ (CO), has a small pore at its center (Fig. 3A, C). A few eutardigrades, such as Doryphoribius dawkinsi Michalczyk & Kaczmarek, 2010 [86] and Ursulinius pappi [32], also possess a CO. The position, different morphology of the cuticle, and the small pore in the centre of this structure are significantly similar to those of the limnoterrestrial echiniscoidean Echiniscus testudo (Doyère, 1840) [24] structure, which is considered to be homologous to the unpaired median cirrus of marine heterotardigrades [20]. Thus, Ramazzottius groenlandensis sp. nov. displays a set of head sensory organs that is most probably identical in origin, position, and function to that of heterotardigrades, i.e., frontal lobes vs. a pair of internal cirri + secondary clavae; AVL vs. a pair of external cirri; EO vs. a pair of lateral cirri + primary clavae; CO vs. unpaired median cirrus (Fig. 9; Table 3). Our findings strongly support the hypothesis that head sensory organs in eutardigrades are homologous to those in heterotardigrades, corroborating the homology between head sensory organs of heterotardigrades and sensory fields of eutardigrades [20].

Fig. 9
figure 9

False-colored scanning electron microscopic images of heterotardigrades and eutardigrades. This figure follows the hypothesis for the homology of head sensory organs and head sensory fields by Gross et al. [20]. A heterotardigrade Echiniscus testudo. B eutardigrade Ramazzottius groenlandensis sp. nov. C eutardigrade Milnesium sp. D eutardigrade Paramacrobiotus areolatus. Colored area and dotted area mean head sensory organs and head sensory fields, respectively. Red: internal cirri & secondary clavae / frontal lobes / anterolateral sensory field (ASF). Blue: external cirri / anteroventral lobes (AVL) / cephalic papillae / ventrolateral sensory field (VSF). Yellow: median sensory field (MED) / centrodorsal organ (CO). Green: cirrus A & primary clava / elliptical organ (EO) / posterolateral sensory field (PSF)

Table 3 Homology of the head sensory structures in the heterotardigrade species Echiniscus testudo and eutardigrade species Ramazzottius groenlandensis sp. nov. and Hypsibius exemplaris, based on Gross et al. [20]

Ramazzottius groenlandensis sp. nov. and Ramazzottius oberhaeuseri species complex

The detailed integrative redescription [31] of Ramazzottius oberhaeuseri established the delimitation criterion of both R. oberhaeuseri sensu stricto and the R. oberhaeuseri species complex: R. oberhaeuseri species complex was defined as a cluster of Ramazzottius species characterized by eggs with hemispherical processes. In addition to R. thulini and R. libycus, the phylogenetic analysis using COI sequences of [31] identified eight potential species within the complex (R. aff. oberhaeuseri sp. 6 was resolved as R. oberhaeuseri sensu stricto). However, based on the COI sequence data from this study, Ramazzottius cf. oberhaeuseri species 2 from [31] has been reclassified as Ramazzottius groenlandensis sp. nov. Since the eggs of R. groenlandensis sp. nov. possess conical processes rather than hemispherical processes, this suggests that R. groenlandensis sp. nov. is not a member of the R. oberhaeuseri species complex.

Although posterior probability (pp) values appear to be low, the COI-based phylogenetic analysis conducted in this study (Fig. 8) presents nine potential Ramazzottius species, a conclusion further supported by the results obtained from pairwise genetic distance calculations and ASAP analysis (Supplementary Data 3). Within these nine species, six species of the R. oberhaeuseri complex (R. aff. oberhaeuseri species 1, 3–5, 7, 8) as identified in the previous study [31] are also recovered. Notably, despite the modest pp values, the tree suggests that (R. aff. oberhaeuseri species 7 + R. aff. oberhaeuseri species 8) forms a sister group to all other Ramazzottius species, excluding R. kretschmanni. Moreover, the 18S + 28S + COI phylogenetic analysis (Fig. 7) also indicates that R. aff. oberhaeuseri species 8 forms a group with (R. oberhaeuseri + R. varieornatus + R. groenlandensis sp. nov.). Therefore, R. oberhaeuseri species complex is not supported phylogenetically. This implies that R. oberhaeuseri group is likely to be a morphogroup, rather than a species complex.

Distribution of Ramazzottius groenlandensis sp. nov.

Ramazzottius groenlandensis sp. nov. displays a notably extensive distribution range spanning from Greenland and Svalbard to Italy, with the most distant localities being approximately 4,000 km apart. However, it appears that R. groenlandensis sp. nov. predominantly occupies specific environments. Notably, the three localities where the species was found are characterized by polar and mountainous conditions, strongly suggesting a preference for cold environments. This inclination aligns with similar tendencies observed in other several tardigrade lineages, such as Cornechiniscus holmeni, Bertolanius, Macrobiotus ariekammensis, and Cryoconicus [87,88,89,90]. Recently, an experiment has proposed phoresis, particularly involving snails, as a means of short-distance terrestrial tardigrade dispersal [91]. However, the mechanism by which terrestrial tardigrades can disperse over such long distances remains unresolved, as both birds and wind have been proposed as possible dispersal vectors [90].

Conclusion

The integrative description of Ramazzottius groenlandensis sp. nov. provided insights into the evolution of the head sensory organs within tardigrades. The correspondence of seven head structures of R. groenlandensis sp. nov. to the cephalic cirri and clavae of heterotardigrades supports the homology of head sensory organs between heterotardigrades and eutardigrades. This result suggests that the last common ancestor of Eutardigrada could have possessed the set of sensory organs on the head. Furthermore, the results of molecular analyses imply that the R. oberhaeuseri group is likely to be a morphogroup, rather than a species complex.

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request. This article has been registered at Zoobank (https://zoobank.org/5F851FDE-7017-4244-A257-46AF8FD983AA).

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Acknowledgements

The authors would like to thank Piotr Gąsiorek and an anonymous reviewer for their valuable comments and corrections. We thank to Denis Tumanov (Saint Petersburg State University) for his comments on the Ramazzottius rupeus. Sampling was carried out under the Greenland government permission number: SP-19-2019.

Funding

This work was supported by Korea Polar Research Institute (KOPRI) grant funded by the Ministry of Oceans and Fisheries (KOPRI project No. PE23060).

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JHK, HSR, and TYSP designed the study. JHK and TYSP collected the samples. JHK performed the experiments and analysed the data. JHK and KZ wrote the first draft of the paper. JHK, KZ, HSR, and TYSP revised and edited the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ji-Hoon Kihm.

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Supplementary Information

Additional file 1:

  Supplementary Fig. 1. The study area of this study: Ella Island.

Additional file 2:

  Supplementary Table 1. Primer information for genotyping.

Additional file 3:

  Supplementary Table 2. PCR condition for this study.

Additional file 4:

  Supplementary Data 1. GenBank accession numbers of the 18S, 28S, and COI sequences used for the 18S + 28S + COI phylogenetic analysis of this study (see Fig. 7).

Additional file 5:

  Supplementary Data 2. GenBank accession numbers of the COI sequences used for the COI phylogenetic analysis of this study (see Fig. 8).

Additional file 6:

  Supplementary Data 3. Pairwise genetic distances of COI, 18S, and 28S, and ASAP result.

Additional file 7:

  Supplementary Data 4. Raw measurement data of Ramazzottius groenlandensis sp. nov.

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Kihm, JH., Zawierucha, K., Rho, H.S. et al. Homology of the head sensory structures between Heterotardigrada and Eutardigrada supported in a new species of water bear (Ramazzottiidae: Ramazzottius). Zoological Lett 9, 22 (2023). https://doi.org/10.1186/s40851-023-00221-w

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