Evolution of the vertebrate skeleton: morphology, embryology, and development
© Hirasawa and Kuratani; licensee BioMed Central. 2015
Received: 16 January 2014
Accepted: 19 February 2014
Published: 13 January 2015
Two major skeletal systems—the endoskeleton and exoskeleton—are recognized in vertebrate evolution. Here, we propose that these two systems are distinguished primarily by their relative positions, not by differences in embryonic histogenesis or cell lineage of origin. Comparative embryologic analyses have shown that both types of skeleton have changed their mode of histogenesis during evolution. Although exoskeletons were thought to arise exclusively from the neural crest, recent experiments in teleosts have shown that exoskeletons in the trunk are mesodermal in origin. The enameloid and dentine-coated postcranial exoskeleton seen in many vertebrates does not appear to represent an ancestral condition, as previously hypothesized, but rather a derived condition, in which the enameloid and dentine tissues became accreted to bones. Recent data from placoderm fossils are compatible with this scenario. In contrast, the skull contains neural crest-derived bones in its rostral part. Recent developmental studies suggest that the boundary between neural crest- and mesoderm-derived bones may not be consistent throughout evolution. Rather, the relative positions of bony elements may be conserved, and homologies of bony elements have been retained, with opportunistic changes in the mechanisms and cell lineages of development.
“Is histological development as complete a test of homology as morphological development?” (Huxley, 1864 : 296)
The vertebrate skeletal system has paramount importance for analyses in evolutionary biology. Because vertebrate skeletons can be viewed as aggregates of apparently discrete units, namely bones, they have attracted the interest of comparative anatomists since even before the dawn of the concept of evolution . In addition, because bones can be preserved as fossils, comparative research can include extinct vertebrates, thereby shedding light on evolutionary patterns and processes (e.g., ). In addition, the vertebrate skeletal system is well suited to biomechanical analyses, allowing both morphological and functional transitions throughout evolution to be reconstructed (e.g., ).
In any comparative study, homology is a conceptual basis for comparing equivalent units. There is, however, a difficulty in establishing homology—that is, “the apparent loose relationship between morphological characters and their genetic basis” . Incongruities between morphologies and their genetic bases may lead to errors when homology is defined solely according to criteria of ontogeny.
Skeletal systems of vertebrates are intolerant of such incongruities (reviewed by ). Historical continuities of skeletal elements as step-wise morphological changes along a phylogenic lineage are inferable from detailed comparative analyses. However, within these continuities, discontinuities of genetic and developmental bases arise in which morphologically homologous bones are produced through different developmental processes [7,8].
Before the concept of evolution was established, two distinct types of bones were recognized in vertebrate skeletons and were thought to reflect their embryonic development; specifically, whether the bone arose from a cartilaginous precursor or not (e.g., [9,10]). Bone arising from precursor cartilage develops not only on the surface of the cartilage (perichondral ossification), but also within the cartilage mass as the cartilage template becomes degraded (endochondral ossification), thereby distinguishing this type of bone from that lacking a cartilaginous precursor in terms of developmental process, or histogenesis. This line of demarcation in histogenesis was later considered to reflect the evolutionary succession of bones. For example, Huxley (1864: 298)  wrote, “It is highly probable that, throughout the vertebrate series, certain bones are always, in origin, cartilage bone, while certain others are always, in origin, membrane bone.” In addition, differences in the cell type of the osteoblast precursors—either mesodermal or neural crest cells—has historically been offered in support of the notion that these two histogenetically distinct types of bone generally evolved separately. However, here, we confirm, through a review of both classical and recent research, that both histogenesis and cell lineage are decoupled with the two independent lineages of skeletal systems, namely endo- and exoskeletons, the continuities of which are inferable from comparative morphology.
In this review, we first summarize various evolutionary continuities of vertebrate skeletal systems. We then describe their developmental bases at two hierarchal levels, namely histogenesis and cell lineage, according to recent studies in developmental biology. In light of this understanding, we discuss the loose relationship between morphology and developmental basis and suggest that a frame shift in character identity occurred across cell lineages during the evolution of vertebrate skeletal systems.
Morphological divisions—endoskeleton vs. exoskeleton
Classification of skeletal systems
Vertebrae, ribs, limb bones
Centra of teleosts, sesamoid, orbitosphenoid of the Amphisbaenia
Skull roof bones, dentary, clavicle, gastralia, scale of fishes, osteoderm
There is convincing evidence that cartilaginously preformed bones changed during evolution to become intramembranous bones. For example, the orbitosphenoid, a cranial skeletal element, of the Amphisbaenia (Reptilia: Squamata) develops intramembranously, although it clearly is homologous with the cartilaginously preformed orbitosphenoid of other tetrapods . Patterson (1977)  proposed calling such intramembranous bones “membrane bones” and discriminated them from bones that developed within the dermis, or “dermal bone.” According Patterson’s terminology, the endoskeleton consists of cartilage and membrane bones (Table 1: Note that the above-mentioned Huxley’s definition of “membrane bone” is different from that used in this paper, as he did not distinguish dermal bones from other intramembranously formed bones).
In contrast, the exoskeleton consists of dermal bones (sensu ), which are homologous with bony armor and are often coated with enameloid or dentine tissues in basal vertebrates (Figure 1, Table 1; ). Exoskeletal bones are located superficially in the body in ancestral conditions, but some exoskeletal bones, such as the dentary and clavicle of mammals, have shifted in their positions to a layer deeper than that of some muscles [18-20]. In this sense, the dentary and clavicle might be referred to as “sunken exoskeleton.”
The previously mentioned evolutionary shifts in the topographic positions of exoskeletal elements recalls the idea of Holmgren (1940) , who suggested that, in some cases, various exoskeletal elements evolved into endoskeleton as the result of a topographic shift (delamination theory). However, studies of comparative morphology provide no evidence of interchangeability between endo- and exoskeletons ; the two historical lines of endo- and exoskeletal systems are likely to have evolved quite independently from each other. It is true that, in some cases, exposed endo- and exoskeletal elements become fused into a single element during ontogeny, as seen in the ontogenetic fusion between endoskeletal costal plates and exoskeletal peripherals to form the carapace in turtles, and in the fusion between endoskeletal vertebrae and exoskeletal osteoderms to form a tail club in ankylosaurid dinosaurs . However, the ossification centers maintain their separate entities, implying incompatibility between the endo- and exoskeletons. (Nevertheless, it is worth noting that a vestigial component of the cleithrum (exoskeletal element) on the scapula (endoskeletal element) in mammals has been suspected repeatedly [29,30]. This evolutionary change represents a “phylogenetic fusion” advocated by Patterson, 1977 ).
Some skeletal elements cannot always be traced back to the ancestral endo- or exoskeleton. There are some examples of newly acquired endo- or exoskeletons in various derived taxa. In special cases, bones are sometimes produced within musculotendinous tissues as neo-formations in specific taxa (e.g., the ossified tendon ; and sesamoid bones) or by pathologic ossification. Smith (1947)  called these bones “subdermal bones,” whereas Patterson (1977)  classified them as membrane bones and components of the endoskeleton (Table 1).
As another example of newly evolved endoskeletal bones, the baculum is a cartilage bone that was newly acquired in the lineage of eutherian mammals . Likewise, non-eutherian mammals have epipubic bones, which were newly acquired in the more basal mammalian lineage and lost in the crown eutherians . It remains uncertain whether the baculum evolved from the epipubic bone of non-eutherian mammals , but examples of the baculum and epipubic bone are suggestive of a novel cartilage bone (a component of the endoskeletal system) that was acquired as an autapomorphy of a specific clade.
In addition, novel exoskeletal elements have been acquired in specific clades. The predentary and rostral bones are examples of such exoskeletal elements [36,37]. Osteoderms (the bony plates covering body contours) occur recurrently throughout vertebrate evolution [38-40]. Although morphological traits are distributed intermittently along the phylogeny, osteoderms are considered to share a developmental basis (“latent homology” sensu ), perhaps illustrative of the historical continuity of these bony elements [39,40].
Histogenesis: endochondral and intramembranous ossifications
In contrast to the distinction of exo- and endoskeletons, adjectives such as ‘endochondral’, ‘dermal’ and ‘intramembranous’ are used exclusively for histogenetic aspects of skeletal tissues, and primarily unrelated to skeletal morphological identities . In many cases, endoskeletal bones develop in association with preexisting cartilage, whereas exoskeletal bones develop solely intramembranously. However, some endoskeletal bones develop solely intramembranously, without any association with cartilage (membrane bones: Table 1), and some exoskeletal bones are likewise associated with cartilage. Comparative morphology studies have shown that cartilaginously preformed bone in the ancestral endoskeleton became intramembranously developed bone in derived taxa (e.g., the orbitosphenoid of the Amphisbaenia ). In contrast, cartilage (secondary or adventitious cartilage) develops on the periphery of exoskeletal bones that develops intramembranously, late in ontogeny of derived clades [7,41]. Cartilage has even been identified in the exoskeletal armor of the trunk (placodont sauropterygians ). Therefore, histogenetic modes with respect to the association of cartilage are interchangeable throughout evolution, as once suggested by De Beer (1937) .
Cartilaginously preformed bone is produced through both intramembranous (perichondral) and endochondral ossification. In perichondral ossification, the typical mode for periosteal bone formation, osteoblasts are differentiated from the perichondrium/periosteum surrounding the cartilage and subsequently produce the osteoid inside the periosteum. In the development of the costal plate of the turtle carapace, the periosteum expanded outward; therefore, osteoblasts produce outgrowths of the periosteal bone collar, or bony trabeculae .
According to histological analyses of fossils, perichondral ossification evolved in the clade containing osteostracans and jawed vertebrates, whereas the endoskeletons of galeaspids comprise calcified cartilages, not perichondral bones . Endochondral ossification originated evolutionarily in osteichthyes—that is, later than the emergence of perichondral ossification .
In the development of the cranial exoskeletal bones of extant osteichthyans, osteogenic cells are differentiated from mesenchymal condensations in the dermis. During this process of intramembranous ossification, osteoblasts mature from a specific transitional cell type (chondrocyte-like osteoblast), which co-expresses both osteogenic and chondrogenic marker genes .
Postcranial osteoderms (exoskeletal bones) develop in the dermis, presumably regulated by an intimate interaction with the epidermis. For example, in armadillos, the osteoderm is produced by osteoblasts that are differentiated from the condensation of dermal cells, with the orientation of the primordial osteoderm parallel to that of the epidermis . In contrast, the osteoderm of alligators develops beneath the keel of scutes, but no osteoblasts are morphologically recognizable during this process . There remains much room for investigation regarding the development of reptilian osteoderms.
In some fishes, exoskeletal bones are coated with enameloid or dentine tissues, namely, odontogenic components (reviewed by ). These enameloid- and dentine-coated bones occur widely among stem-osteichthyans, and odontogenic components are present in chondrichthyans also. However, the odontogenic components seen in chondrichthyans are believed to represent the vestige of the enameloid- and dentine-coated bones of ancestral jawed vertebrates, in which the bony portion was lost secondarily —the exoskeleton of stem-gnathostomes likely was composed primarily of bone. This view is supported by recent histological data from placoderms (a taxon of stem-gnathostomes), indicating that the condition seen in extant chondrichthyans is derived. In placoderms, bony components always contributed to the exoskeleton, whereas odontogenic components did not always contribute to the exoskeleton [52,53], suggesting that odontogenic components were not prerequisite for exoskeletal development in these taxa.
In addition to endochondral and intramembranous ossifications there is a disparate mode of bone formation, namely metaplastic bone formation , the process by which preexisting tissues change directly (i.e., through metaplasia) into bony tissues. Exposed endoskeleton [12,55,56] and exoskeleton  contain portions of metaplastic bone, in which the collagen fibers of the dermis are engulfed.
Developmental origins and cell lineages—mesoderm and neural crest in the vertebrate cranium
Developmentally, the skeletal tissues of vertebrates have dual origins—the mesoderm and neural crest. Platt (1893)  suggested that the ectodermally derived mesenchyme (that is, ectomesenchyme) contributes to the cranial skeleton in basal vertebrates. De Beer (1958, 1971) [61,62] later used Platt’s notion to refute von Baer’s germ layer theory , because mesoderm generally was believed to be the main source of skeletal tissue in animals.
In the context of comparative embryology and morphology, the cranium traditionally has been divided into several components, primarily the dorsal and ventral moieties (the neurocrania and viscerocrania, respectively) [43,79,87-94]. The neurocrania and viscerocrania are both recognized as endoskeletons over which a dermal covering, the dermatocranium, develops to encapsulate the entire endocranium. As noted earlier, the endocranium forms as a cartilage precursor and either ossifies through endochondral ossification to be replaced by bone, or degenerates (in cases in which dermal bones can perform the same functions). The cartilaginous skull roof in elasmobranchs is complete, but in animals in which the dermal skull roof is well developed that part of the cartilaginous neurocranium typically is absent.
Like the cranium, the dermatocranium can be divided into dorsal and ventral components corresponding to its neural and visceral elements. The cartilaginous neurocranium was initially recognized as a rostral continuation of the vertebral column, its elements being united and expanded to hold the enlarged brain. In contrast, the viscerocranium is composed of serial and metameric visceral arch skeletons surrounding the pharynx. In jawed vertebrates, one of the rostral elements is enlarged and divided dorsoventrally into the upper and lower jaws. The developmental origins of these cranial components have been, and remain, the focus of much debate.
According to Noden (1988)’s scheme , the neural crest-derived ectomesenchyme resides predominantly within the ventral part of the pharyngular head, in the region in which the craniofacial structures will form, whereas the majority of the cranial mesoderm is found more dorsally, lateral to the notochord and surrounding the brain primordium  (reviewed by ). This arrangement prompts the speculation that the distinction between neurocrania and viscerocrania will correspond to that of their embryonic cell lineages, i.e., mesoderm and neural crest. This seems reasonable, given that, like that of trunk somites, chondrification of the mesoderm is understood to require signals that emanate from the notochord. In contrast, the skeletogenesis of neural crest cells differs from that of the paraxial mesoderm, and is highly dependent on epithelial–mesenchymal interactions  (reviewed by ). Although this explanation holds true for part of the cranium, it is contradicted elsewhere.
The dermal elements of the calvarium are likely patterned according to the lateral line system, and thus the homology of these elements is, in aquatic forms, based on the homology of lateral lines (see [59,114] and references therein; Figure 7C–F). Although the patterns of dermal bones and lateral lines are coupled developmentally, it is unclear whether the lateral line induces the dermal bones, or vice versa (see ). Presumably the typical dermal bones found in fishes (including placoderms) became secondarily sunken exoskeletal elements concomitant with the shift in developmental interactions to induce membranous ossification in a deeper layer of the dermis, as found in amniotes. Questions remain regarding homologies (evolutionary continuities) of the dermal elements (reviewed by ), as well as their early evolution. The pattern of the dermal skull roof perhaps was first established in placoderms  (Figure 7B–F; also see ), in which the topographic relationship between dermal bones and lateral lines seen in modern vertebrates is recognizable, at least in part. From lines of circumstantial evidence regarding neural crest contribution and its putative relationship with lateral lines, it is unlikely that the dermal skull roof elements represent segmental organization of the vertebrate head. The lateral lines are not induced as primordia with any segmental prepatterning (for the developmental pattern of the placodes, see  and references therein); therefore, the dermal skull roof elements may form independently of any segmental prepattern.
By constructing chick–quail chimeras, Noden found that the rostral part of the dermal skull roof is derived from the neural crest, whereas the posterior arises from the mesoderm [80,81,118,119] (Figure 5A). The boundary between these two cell lineages lies in the frontal bone (for the homology of the avian frontal bone, see ). Similar results from a similar experiment were obtained by Le Lièvre (1978) . However, Couly et al. (1993)  showed that the entire dermis, as well as the dermatocranial elements, is exclusively of neural crest origin (Figure 5B). To date, systematic fate mapping of the avian craniofacial structures has not been completed; the explanation underlying these inconsistent results remains unclear, but may involve contamination by non-crest tissues or incomplete postsurgical wound healing (summarized by ).
Regardless, the views of Couly et al. (1993)  once prevailed among zoologists and carried the expectation that the entire exoskeleton of vertebrates—head and trunk—would be of neural crest origin (reviewed by ). Another finding that appeared to strengthen this assumption was that the differentiation repertoire of the neural crest is not entirely predetermined differentially along the anteroposterior axis (head versus trunk); heterotopically transplanted trunk neural crest can exhibit skeletogenic potency in the head environment of the embryo  (also see  for a similar experiment; also see ). It was thus speculated that the trunk neural crest is normally suppressed from differentiating into the exoskeleton in animals that have lost most of the postcranial exoskeleton, which, however, can be reactivated under specific circumstances. In fact, all exoskeletal elements in vertebrates, including the dermal skull roof, teleost scales, lepidotrichia, and the extensive head shield in some fossil lineages such as osteostracans and placoderms, were expected to originate from the neural crest —despite the lack of any supporting evidence for this notion. This overly simplified prediction was further extended to postulate the involvement of the neural crest in the turtle shell, which had often been interpreted erroneously as an exoskeletal element (see ; see above). Here, the mesoderm-crest duality was related to an in–out topography of endo/exoskeletal parts in the neurocranium, not along the dorsoventral axis.
New embryonic technologies have apparently dispelled the above unsubstantiated assumptions. Shimada et al. (2013) , for example, performed transplantations of somites and neural crest in medaka embryos and convincingly showed that the trunk scales of this fish originate from the mesoderm, not the neural crest. Analyses of transgenic lines of zebrafish by several other groups yielded similar results [75,125]. However, several groups suspect that the neural crest contributes to the exoskeleton of the trunk, for example, to the lepidotrichia of the caudal and dorsal fins in zebrafishes  and the turtle plastron [124,126]. Furthermore, a recent study tracing the lineages of transgenic cells revealed that trunk neural crest cells do not generate a skeletogenic tissue (that is, ectomesenchyme)  although they have skeletogenic potential in the developing head . These lines of evidence, in combination with the fossil evidence from placoderms [52,53] (see above), suggest that the exoskeleton of the trunk develops from the mesoderm in the ancestral condition in the jawed vertebrates and that accretions of the enameloid and dentine tissues (i.e., odontogenic component) to the trunk exoskeleton occurred in many lineages, distinct from what had previously been hypothesized (e.g., ).
Consequently, the interface between the neural crest- and mesoderm-derived parts of the exoskeleton again appears to be somewhere in the skull roof, and different results regarding its specific location have been obtained via different experimental methods in embryos of different taxa (reviewed by ; Figure 5A, B). Our current understanding regarding the origin of vertebrate skull roof is therefore confused.
Transgenic technology was used to label crest-derived ectomesenchyme and its derivatives in mice (Figure 5C) [30,70,72,83]. In this model, the Wnt-1 promoter was used to drive Cre to activate a reporter gene as a marker for all neural crest cells. This methodology resulted in labeling of the nasal, frontal, and interparietal regions in addition to the more ventrally located dermal elements, and the signal distribution was complementary to the pattern obtained by using Mesp1-Cre/R26R to label mesodermal cell lineages (see ). This result resembles those of Noden (1978, 1982, 1983, 1984) [80,81,118,128] and Le Lièvre (1978)  in avian embryos (Figure 5A; Evans and Noden, 2006 , subsequently confirmed these previous results by labeling mesoderm through retroviral infection). Furthermore, these current and previous findings coincide perfectly if we admit misidentification of the boundary between the frontal and parietal regions in mammals and avians: the supraoccipital region is the dorsal portion of a mesodermal element serially homologous with the vertebrae, and the interparietal region may not be present in avians (for the homology and evolution of the interparietal region, see  and references therein).
Morphological homologies of bony elements and the cell lineages that give rise to these elements are regulated at different, decoupled levels, and the bony elements can be conserved through evolution independent from the cell lineages, which are apt to change more rapidly.
The ancestral developmental pattern and cell-lineage origins of the dermatocranial elements were established in various fossil taxa, which are reflected in some modern taxa, and are secondarily modified in others, possibly because of the loss or fusion of ancestral elements or the addition of new elements.
The dermatocranium (excluding the supraoccipital region) primarily was derived from the cranial neural crest ancestrally, and new mesodermal elements intercalated secondarily to accommodate adaptation to the expansion of the cranial vault in different ways in each animal lineage, obliterating homologies between some bones (as suggested in Figure 7, the parietal bone represents a newly inserted mesodermal element).
The dermatocranium (excluding the supraoccipital bone) was primarily derived from the mesoderm ancestrally, and new crest-derived elements were intercalated secondarily to accommodate adaptation to the expansion of the cranial vault in different ways in each animal lineage, thus obliterating homologies of bones.
The pattern of dermal elements belongs to most variable parts of the vertebrate body, and developmental constraints assure homologies of dermal elements only within limited levels of taxa (orders, superfamilies, etc.; see [135-137]; reviewed by ).
Mesodermal dermal elements were associated primarily with various lateral lines in ancestral forms, and other elements were all derived from the neural crest (Figure 5D and F).
The lateral line-induced dermal elements in ancestors have been lost, and the tetrapod dermatocranium, predominantly derived from the neural crest, has been newly reorganized in each animal lineage in its unique way.
None of the above scenarios has been assessed experimentally to date, nor have discrepancies among experimental embryologic data been reconciled. According to the third scenario, the parietal would have to be regarded as a synapomorphy in crown gnathostomes, which however, may be refuted by the fact that the majority of placoderms possess this bone .
The situation may be even more confusing than that presented. If the apparent inconsistency in the mesoderm–neural crest boundary could be explained, it may turn out to be attributable to a misnaming of bony elements; this could be resolved by morphological and developmental reexamination of homologous relationships . Unfortunately, however, this confusion may be destined to be insurmountable. Transgenic and chimeric approaches have revealed that the cranium of the frog violates generally accepted rules of development—that is, the developmental origins of the visceral arch and craniofacial skeletons are not found in a canonical set of crest cell streams that are divided into mandibular, hyoid, and branchial arch streams; instead, morphologically homologous dermal elements are derived from inconsistent cell lineages in frog embryos (Figure 6D-F) [111,138]. Therefore, the skeletal development of the frog demonstrates the decoupling of embryonic patterns, cell lineages, and adult morphology in a very radical manner. It is conceivable that, especially in animals that go through metamorphosis, insertion of larval stages causes topographical shifts of the neural crest-derived chondrogenic cells that go on to form adult skeletons (although this does not explain the hyoid crest-origin of the prechordal cranium in amphibians as reported by Olsson and Hanken (1996) ). The same may be the case in the development of the dermal skull roof; the morphological patterns and homologies may reside in the local environment of the embryos, such that they become specified during a later phase of development. This potential influence of the local environment recalls the study of Schneider (1999) , in which cranial neural crest from the quail embryo was ectopically grafted within mesenchymal populations destined to form the skull wall in the chicken embryo. In resulting chimeras, these grafted cells gave rise to a skeletal element, which in birds is normally derived from the mesoderm. There are many more examples that demonstrate the importance of local tissue interaction in the specification of bony elements [128,140] (also see a review by ) by showing potential shifts of cell lineages and populations to generate morphologically conserved skeletal patterns during evolution.
A similar situation is seen in the apparent discrepancy of the neural crest contributions to the pectoral girdle bones between amniotes [30,141] and anamniotes [73,142]. It is generally accepted that, within the mesodermal cell population, the developmental basis providing the skeletal identities of the digits shifted between non-homologous primordia in the evolution from dinosaurs to birds (frame-shift hypothesis) [143-145]. No accounts contradict the possibility that skeletal identities similarly shift between neural crest and mesodermal cell populations.
Perspectives—beyond the complexity
The vertebrate skull initially attracted the attention of zoologists because of its complex and elegant morphology, but its complexity clearly exceeds all expectations. Theories regarding skeletogenesis and skeletal anatomy and its evolution have been—and still are—fraught with confusion, which never seems to be resolved easily. This situation cannot be ascribed only to the misuse of terminology in non-comparable contexts of discussion; it also reflects the complexity of the developmental and evolutionary diversity of the vertebrate skeletal system per se. Nor is the current developmental understanding of skeletogenesis formulated in an orderly way into the pattern of embryos and cell lineages.
The dilemma described here is tightly linked to the confusion regarding the concept of homology. As noted earlier, morphological homology was in the past reduced to its developmental origins in cell lineages and germ layers, as seen in von Baer’s germ layer theory (reviewed by ). This theory was refuted as being based on inaccurate concepts of histogenesis, including the concept that skeletogenic differentiation can take place equally in mesodermal and ectodermal (neural crest) cell lineages. Still, the neural crest – mesoderm distinction, as well as endochondral–membranous ossifications, was expected to coincide with specific morphological components of the skull—a belief that could be viewed as a modified version of the germ layer theory. Alternatively, a similar reductionist argument was once widespread with a vague expectation in the dawn of evolutionary developmental biology; namely, that morphologically homologous structures should be patterned through certain unchanged infrastructures, like function of evolutionarily conserved sets of regulatory genes or gene regulatory networks.
Expectations such as these often come true, as typically exemplified by the isomorphic shifts of vertebral formula and Hox code  (also see ). In this context, the positional identities of vertebrae along the anteroposterior axis of the vertebral column (such as occipital, cervical, thoracic, lumbar, and sacral in mammals) coincide precisely with the expression domains of Hox genes in the prevertebral anlagen, and under this Hox-code-mediated specification the number of segments can vary during evolution (for variable numbers of vertebrae, see ). In this case, morphological homology is reduced to the regulation of homologous Hox genes. Similar situations, in which the homology between structure and gene expression is tightly conserved, include the expression of homeobox genes and primordial segments in the developing vertebrate brain, differentiation of somite-derivatives, and dorsoventral specification of the neural tube (reviewed by ). In an extreme reductionist argument that is focused on genes, cell-type identities, which are classified by transcript repertoire (that is, molecular fingerprinting of cell types), are comparable among phyla, even between the vertebrate- and annelid body plans, for example, at the level of single neurons .
Unfortunately, relationships among homologies at different hierarchal levels—namely at the levels of morphology, histogenesis, cell lineage and genes—remain murky, as homologous skeletal elements can arise from different or shifted cell lineages throughout evolution by means of different mechanisms of development, thus challenging the criteria for morphological homology (e.g., [5,150,151]; reviewed by ). Inconsistency of this type occurs in various phenomena of organogenesis, in which homologous structures are patterned by the actions of non-homologous regulatory genes in each animal lineage [153,154]. In the evolutionary context, there are at least two significant effects worth considering.
One effect is evolutionary novelty and simultaneous loss of homology: the shift in developmental interactions in time and place result in novel regulation of skeletogenic genes, leading to a skeletal pattern incomparable to that in the ancestor. The other effect is developmental drift: the developmental process and mechanisms would shift without changing the readout of the shifted developmental process, thus maintaining the ancestral morphological pattern in the adult. De Beer (1958)  noted the heterochronic factor behind similar phenomena, for example, in the creation of the larval stage in development. One of the most conspicuous examples is found in the columella auris (that is, hyomandibular bone) of certain frogs. In Xenopus, the anlage of the columella never appears during the larval stage, but arises during metamorphosis [155,156]. In the mouse, the stapes (the homologue of this cartilage bone) is patterned during embryogenesis in the dorsal part of the second pharyngeal arch and is specified through the upregulation of Hoxa2  in the ectomesenchyme. In Xenopus, homology of this skeletal element appears to be maintained—albeit decoupled from the Hox code— and its differentiation is even suggestive of new involvement of the thyroid hormone in the rewired regulatory network. We have already seen, in frog development, how morphologically homologous cranial elements arise from cell populations or pharyngeal arches not identical to those in other vertebrate groups.
It is true that the morphological homology of skeletal elements cannot be reduced directly to the developmental program, or homology of genes, involved in the generation of homologous structures. However, insofar as the criteria for homology largely rest on the relative positions of organs (reviewed by ), developmental patterns may, to some extent, explain the impetus behind the manifestation of the homologous patterns. This explanation is especially plausible given that the relative positions suggest evolutionarily maintained topography of cell populations and tissues, which act as the bases for embryonic interactions to establish the identities of the skeletal anlagen, especially through the upregulation of specific sets of transcription factor-encoding genes. Here we recall the experiment of Schneider (1999)  to show that neural crest-derived ectomesenchyme and cephalic mesoderm can be exchanged to generate morphologically normal chondrocranium. This experiment indicates that the developmental factor(s) for the morphological homology resides in the “position” in the embryo, not in the embryonic cell lineages. Consistently, a same set of gene expressions has been detected in endochondral ossifications of mesenchymal condensations both derived from neural crest and mesodermal cells . This implication stands in conspicuous contrast to the fact that species-specific shape appears to evolve in the developmental program associated with specific cell lineages [130,133].
To understand the mechanistic background for the burden of development, we have to understand how selective pressure—especially stabilizing selection—at the phenotypic level (adaptation) acts on the developmental program exerted from the genome. In other words, we must identify parts or elements of the developmental program (for example, gene regulatory networks, modules, sets of regulatory genes and their regulatory elements) that can or cannot change when certain fixed phenotypic patterns are favored. These efforts will uncover the aspects of the developmental program that are resistant to change and those that are apt to change during evolution. In evolution, adaptation and constraint cannot be discriminated a priori [158,159]. The key to discriminating between these two causal relationships behind evolution is provided abductively through historical and experimental analyses of the correlation between phenotype and the developmental program behind it (for example, skeletal elements can be considered as a phenotype of a skeletal system). The patterns that allow minimal shifts have been recognized to result from developmental constraint. The concept of developmental constraint has not yet been explained thoroughly, but taxon-specific conserved patterns of embryogenesis have been recognized as the so-called “phylotype,” which tends to appear in the organogenetic stage of development (“phylotypic stage” ).
In transcendental morphology, the phylotype (pharyngula in vertebrates) has been viewed as an embodiment of the conceptual archetype, a shared morphology of the embryos of animals belonging to the vertebrates, from which various types of adult morphologies can be derived . This “derivation,” however, does not necessarily refer to the phylogenetic evolutionary process, but rather to observers’ perceptions of homologous patterns and their developmental changes. Morphologically, it is true that the pharyngula-stage embryo in vertebrates is the stage at which the basic body plan, or a set of homologous anlagen, of this animal group becomes established. In the evo-devo context, the phylotypic stage of vertebrate development is recognized as the stage at which so-called tool-kit genes (typically the Hox code) are expressed most conspicuously during development, thus providing the mechanistic bases to explain the significance of this conserved embryonic pattern . Recent transcriptome analyses have shown that the most similar gene expression profiles coincide with the phylotypic stage . Importantly, as indicated by genomic analyses of turtles, the evolutionarily novel patterns of the skeletal system in vertebrates appear to arise through spatiotemporal developmental shifts after the establishment of the above-noted phylotype . Taking into consideration the shifts in morphological homologies—specifically the developmental patterns and processes involved in patterning of the evolutionarily fixed patterns of craniofacial elements—it seems likely that the cranial pattern is specified late relative to the specification of the phylotype. This delay suggests the presence of another developmental constraint, which is more or less uncoupled from those needed to maintain the phylotype. The search for such taxon-specific constraints, as well as their mechanistic importance, is an intriguing focus for future evo-devo studies. The results likely would further our understanding of the synapomorphies used in the reconstruction of evolutionary history.
We thank Ruth Elsey and Neil Shubin for the gift of American alligator embryos, and Dai Koyabu for critical reading of the manuscript and valuable discussions. We also thank the two anonymous referees for comments that improved the manuscript.
- Huxley TH: Lectures on the elements of comparative anatomy. London: J. Churchill and Sons; 1864.Google Scholar
- Goethe JW: Schädelgrüst aus sechs Wirbelknochen aufgebaut. Zur Morphologie, Band 2, Heft 2. Stuttgart: J. G. Cotta; 1824.Google Scholar
- Simpson GG: Tempo and Mode in Evolution. New York: Columbia University Press; 1944.Google Scholar
- Baier DB, Gatesy SM, Jenkins FA: A critical ligamentous mechanism in the evolution of avian flight. Nature 2007, 445:307–310.PubMedView ArticleGoogle Scholar
- Wagner GP: The developmental genetics of homology. Nat Rev Genet 2007, 8:473–479.PubMedView ArticleGoogle Scholar
- Hall BK: Evolutionary Developmental Biology. 2nd edition. London: Chapman & Hall; 1998.Google Scholar
- Patterson C: Cartilage bones, dermal bones and membrane bones, or the exoskeleton versus the endoskeleton. In Problems in Vertebrate Evolution. Edited by Andrews SM, Miles RS, Walker AD. London: Academic Press; 1977:77–121. Linnean Society Symposium Series.Google Scholar
- Gross JB, Hanken J: Review of fate-mapping studies of osteogenic cranial neural crest in vertebrates. Dev Biol 2008, 317:389–400.PubMedView ArticleGoogle Scholar
- Nesbitt R: Human osteogeny explained in two lectures. London: J. Noon; 1736.Google Scholar
- Arendt E: De capitis ossei Esocis Lucii structura singulari. Regiomonti (Königsberg): Typis academicis Hartungianis; 1822.Google Scholar
- Starck D: Vergleichende Anatomie der Wirbeltiere, Bd. 2. Das Skeletsystem: Allgemeines, Skeletsubstanzen, Skelet der Wirbeltiere einschl. Lokomotionstypen. Berlin, Heidelberg, New York: Springer-Verlag; 1979.Google Scholar
- Hirasawa T, Nagashima H, Kuratani S: The endoskeletal origin of the turtle carapace. Nat Commun 2013, 4:2107.PubMed CentralPubMedView ArticleGoogle Scholar
- Janvier P: Homologies and evolutionary transitions in early vertebrate history. In Major Transitions in Vertebrate Evolution. Edited by Anderson JS, Sues HD. Bloomington: Indiana University Press; 2007:57–121.Google Scholar
- Trinajstic K, Sanchez S, Dupret V, Tafforeau P, Long J, Young G, Senden T, Boisvert C, Power N, Ahlberg PE: Fossil musculature of the most primitive jawed vertebrates. Science 2013, 341:160–164.PubMedView ArticleGoogle Scholar
- Clack JA: Gaining Ground: The Origin and Evolution of Tetrapods. 2nd edition. Bloomington: Indiana University Press; 2012.Google Scholar
- Bellairs AD, Gans C: A reinterpretation of the amphisbaenian orbitosphenoid. Nature 1983, 302:243–244.View ArticleGoogle Scholar
- Smith MM, Hall BK: Development and evolutionary origins of vertebrate skeletogenic and odontogenic tissues. Biol Rev 1990, 65:277–373.PubMedView ArticleGoogle Scholar
- Romer AS: Pectoral limb musculature and shoulder-girdle structure in fish and tetrapods. Anat Rec 1924, 27:119–143.View ArticleGoogle Scholar
- Crompton AW, PD G: On the lower jaw of Diarthrognathus and the origin of the mammalian lower jaw. Proc Zool Soc Lond 1963, 140:697–749.View ArticleGoogle Scholar
- Crompton AW, Parker P: Evolution of mammalian masticatory apparatus. Am Sci 1978, 66:192–201.PubMedGoogle Scholar
- Claessens LPAM: Dinosaur gastralia; origin, morphology, and function. J Vert Paleontol 2004, 24:89–106.View ArticleGoogle Scholar
- Hay OP: On Protostega, the systematic position of Dermochelys, and the Morphologeny of the chelonian carapace and plastron. Am Nat 1898, 32:929–948.View ArticleGoogle Scholar
- Voeltzkow A, Döderlein L: Beiträge zur Entwicklungsgeschichte der Reptilien III.: Zur Frage nach der Bildung der Bauchrippen. Abh Senk Naturforsch Ges 1901, 26:313–336.Google Scholar
- Völker H: Über das Stamm-, Gliedmaßen-, und Hautskelet von Dermochelys coriacea L. Zool Jahrb Anat Ont 1913, 33:431–552.Google Scholar
- Zangerl R: The homology of the shell elements in turtles. J Morphol 1939, 65:383–406.View ArticleGoogle Scholar
- Ferguson MWJ: Reproductive biology and embryology of the crocodilians. In Biology of the Reptilia, Vol 14: Development A. Volume 14: Deveolopment A. Edited by Gans C, Billett F, Maderson PFA. New York: Academic Press; 1985:329–491.Google Scholar
- Holmgren N: Studies on the head of fishes. Part I. Development of the skull in sharks and rays. Acta Zool Stockh 1940, 21:51–267.View ArticleGoogle Scholar
- Hayashi S, Carpenter K, Scheyer TM, Watabe M, Suzuki D: Function and evolution of ankylosaur dermal armor. Acta Palaeontol Pol 2010, 55:213–228.View ArticleGoogle Scholar
- Broom R: On the development and morphology of the marsupial shoulder girdle. Trans Roy Soc Edin 1899, 39:749–770.View ArticleGoogle Scholar
- Matsuoka T, Ahlberg PE, Kessaris N, Iannarelli P, Dennehy U, Richardson WD, McMahon AP, Koentges G: Neural crest origins of the neck and shoulder. Nature 2005, 436:347–355.PubMed CentralPubMedView ArticleGoogle Scholar
- Organ CL: Thoracic epaxial muscles in living archosaurs and ornithopod dinosaurs. Anat Rec 2006, 288A:782–793.View ArticleGoogle Scholar
- Smith HM: Classification of bone. Turtox News 1947, 25:234–236.Google Scholar
- Smirnov DG, Tsytsulina K: The ontogeny of the baculum in Nyctalus noctula and Vespertilio murinus (Chiroptera : Vespertilionidae). Acta Chiropterol 2003, 5:117–123.View ArticleGoogle Scholar
- Novacek MJ, Rougier GW, Wible JR, McKenna MC, Dashzeveg D, Horovitz I: Epipubic bones in eutherian mammals from the late Cretaceous of Mongolia. Nature 1997, 389:483–486.PubMedView ArticleGoogle Scholar
- Jellison WL: A suggested homolog of the Os penis or baculum of mammals. J Mammal 1945, 26:146–147.View ArticleGoogle Scholar
- Sereno PC: The evolution of dinosaurs. Science 1999, 284:2137–2147.PubMedView ArticleGoogle Scholar
- Zhou ZG, Martin LD: Distribution of the predentary bone in Mesozoic ornithurine birds. J Syst Palaeontol 2011, 9:25–31.View ArticleGoogle Scholar
- Hill RV: Integration of morphological data sets for phylogenetic analysis of amniota: The importance of integumentary characters and increased taxonomic sampling. Syst Biol 2005, 54:530–547.PubMedView ArticleGoogle Scholar
- Hill RV: Comparative anatomy and histology of xenarthran osteoderms. J Morphol 2006, 267:1441–1460.PubMedView ArticleGoogle Scholar
- Vickaryous MK, Sire JY: The integumentary skeleton of tetrapods: origin, evolution, and development. J Anat 2009, 214:441–464.PubMed CentralPubMedView ArticleGoogle Scholar
- Bailleul AM, Hall BK, Horner JR: First evidence of dinosaurian secondary cartilage in the post-hatching skull of Hypacrosaurus stebingeri (Dinosauria, Ornithischia). PLoS ONE 2012, 7:e36112.PubMed CentralPubMedView ArticleGoogle Scholar
- Scheyer TM: Skeletal histology of the dermal armor of Placodontia: the occurrence of ‘postcranial fibro-cartilaginous bone’ and its developmental implications. J Anat 2007, 211:737–753.PubMed CentralPubMedView ArticleGoogle Scholar
- De Beer GR: The Development of the Vertebrate Skull. London: Oxford University Press; 1937.Google Scholar
- Maes C, Kobayashi T, Selig MK, Torrekens S, Roth SI, Mackem S, Carmeliet G, Kronenberg HM: Osteoblast precursors, but not mature osteoblasts, move into developing and fractured bones along with invading blood vessels. Dev Cell 2010, 19:329–344.PubMed CentralPubMedView ArticleGoogle Scholar
- Wang NZ, Donoghue PCJ, Smith MM, Sansom IJ: Histology of the galeaspid dermoskeleton and endoskeleton, and the origin and early evolution of the vertebrate cranial endoskeleton. J Vert Paleontol 2005, 25:745–756.View ArticleGoogle Scholar
- Donoghue PCJ, Sansom IJ: Origin and early evolution of vertebrate skeletonization. Microsc Res Techniq 2002, 59:352–372.View ArticleGoogle Scholar
- Abzhanov A, Rodda SJ, McMahon AP, Tabin CJ: Regulation of skeletogenic differentiation in cranial dermal bone. Development 2007, 134:3133–3144.PubMedView ArticleGoogle Scholar
- Vickaryous MK, Hall BK: Osteoderm morphology and development in the nine-banded armadillo, Dasypus novemcinctus (Mammalia, Xenarthra, Cingulata). J Morphol 2006, 267:1273–1283.PubMedView ArticleGoogle Scholar
- Vickaryous MK, Hall BK: Development of the dermal skeleton in Alligator mississippiensis (Archosauria, Crocodylia) with comments on the homology of osteoderms. J Morphol 2008, 269:398–422.PubMedView ArticleGoogle Scholar
- Sire JY, Donoghue PCJ, Vickaryous MK: Origin and evolution of the integumentary skeleton in non-tetrapod vertebrates. J Anat 2009, 214:409–440.PubMed CentralPubMedView ArticleGoogle Scholar
- Romer AS, Parsons TS: The Vertebrate Body. 5th edition. Philadelphia: W. B. Saunders; 1977.Google Scholar
- Downs JP, Donoghue PCJ: Skeletal histology of Bothriolepis canadensis (Placodermi, Antiarchi) and evolution of the skeleton at the origin of jawed vertebrates. J Morphol 2009, 270:1364–1380.PubMedView ArticleGoogle Scholar
- Giles S, Rücklin M, Donoghue PCJ: Histology of “placoderm” dermal skeletons: Implications for the nature of the ancestral gnathostome. J Morphol 2013, 274:627–644.PubMedView ArticleGoogle Scholar
- Haines RW, Mohuiddin A: Metaplastic bone. J Anat 1968, 103:527–538.PubMed CentralPubMedGoogle Scholar
- Scheyer TM, Sánchez-Villagra MR: Carapace bone histology in the giant pleurodiran turtle Stupendemys geographicus : Phylogeny and function. Acta Palaeontol Pol 2007, 52:137–154.Google Scholar
- Scheyer TM, Brüllmann B, Sánchez-Villagra MR: The ontogeny of the shell in side-necked turtles, with emphasis on the homologies of costal and neural bones. J Morphol 2008, 269:1008–1021.PubMedView ArticleGoogle Scholar
- Witzmann F: Comparative histology of sculptured dermal bones in basal tetrapods, and the implications for the soft tissue dermis. Palaeodiversity 2009, 2:233–270.Google Scholar
- Eames BF, Allen N, Young J, Kaplan A, Helms JA, Schneider RA: Skeletogenesis in the swell shark Cephaloscyllium ventriosum . J Anat 2007, 210:542–554.PubMed CentralPubMedView ArticleGoogle Scholar
- Zhu M, Yu XB, Ahlberg PE, Choo B, Lu J, Qiao T, Qu QM, Zhao WJ, Jia LT, Blom H, Zhu YA: A Silurian placoderm with osteichthyan-like marginal jaw bones. Nature 2013, 502:188–193.PubMedView ArticleGoogle Scholar
- Platt JB: Ectodermic origin of the cartilage of the head. Anat Anz 1893, 8:506–509.Google Scholar
- De Beer GR: Embryos and Ancestors. Oxford: Oxford University Press; 1958.Google Scholar
- De Beer GR: Homology, An Unsolved Problem. Oxford: Oxford University Press; 1971.Google Scholar
- von Baer KE: Entwicklungsgeschichte der Thiere: Beobachtung und Reflexion. Born Träger: Königsberg; 1828.Google Scholar
- Hall BK, Hörstadius S: The Neural Crest. New York: Oxford University Press; 1988.Google Scholar
- Hall BK: Bones and Cartilage: Developmental and Evolutionary Skeletal Biology. London: Elsevier Academic Press; 2005.Google Scholar
- Newth DR: Experiments on the neural crest of the lamprey embryo. J Exp Biol 1951, 28:247–260.Google Scholar
- Newth DR: On the neural crest of the lamprey embryo. J Embryol Exp Morph 1956, 4:358–375.Google Scholar
- Langille RM, Hall BK: Role of the neural crest in development of the trabeculae and branchial arches in embryonic sea lamprey, Petromyzon-Marinus (L). Development 1988, 102:301–310.Google Scholar
- Kuratani S, Murakami Y, Nobusada Y, Kusakabe R, Hirano S: Developmental fate of the mandibular mesoderm in the lamprey, Lethenteron japonicum : comparative morphology and development of the gnathostome jaw with special reference to the nature of the trabecula cranii. J Exp Zool B (MDE) 2004, 302B:458–468.View ArticleGoogle Scholar
- Jiang XB, Iseki S, Maxson RE, Sucov HM, Morriss-Kay GM: Tissue origins and interactions in the mammalian skull vault. Dev Biol 2002, 241:106–116.PubMedView ArticleGoogle Scholar
- McBratney-Owen B, Iseki S, Bamforth SD, Olsen BR, Morriss-Kay GM: Development and tissue origins of the mammalian cranial base. Dev Biol 2008, 322:121–132.PubMed CentralPubMedView ArticleGoogle Scholar
- Yoshida T, Vivatbutsiri P, Morriss-Kay G, Saga Y, Iseki S: Cell lineage in mammalian craniofacial mesenchyme. Mech Develop 2008, 125:797–808.View ArticleGoogle Scholar
- Kague E, Gallagher M, Burke S, Parsons M, Franz-Odendaal T, Fisher S: Skeletogenic fate of zebrafish cranial and trunk neural crest. PLoS ONE 2012, 7:e47394.PubMed CentralPubMedView ArticleGoogle Scholar
- Le Douarin N: The Neural Crest. Cambridge: Cambridge University Press; 1982.Google Scholar
- Lee RTH, Thiery JP, Carney TJ: Dermal fin rays and scales derive from mesoderm, not neural crest. Curr Biol 2013, 23:R336–R337.PubMedView ArticleGoogle Scholar
- Lee RTH, Knapik EW, Thiery JP, Carney TJ: An exclusively mesodermal origin of fin mesenchyme demonstrates that zebrafish trunk neural crest does not generate ectomesenchyme. Development 2013, 140:2923–2932.PubMed CentralPubMedView ArticleGoogle Scholar
- Shimada A, Kawanishi T, Kaneko T, Yoshihara H, Yano T, Inohaya K, Kinoshita M, Kamei Y, Tamura K, Takeda H: Trunk exoskeleton in teleosts is mesodermal in origin. Nat Commun 2013, 4:1639.PubMed CentralPubMedView ArticleGoogle Scholar
- Noden DM: Interactions and fates of avian craniofacial mesenchyme. Development 1988, 103:121–140.PubMedGoogle Scholar
- Kuratani S: Craniofacial development and the evolution of the vertebrates: the old problems on a new background. Zool Sci 2005, 22:1–19.PubMedView ArticleGoogle Scholar
- Noden DM: Patterns and organization of craniofacial skeletogenic and myogenic mesenchyme: a perspective. Prog Clin Biol Res 1982, 101:167–203.PubMedGoogle Scholar
- Noden DM: Craniofacial development: new views on old problems. Anat Rec 1984, 208:1–13.PubMedView ArticleGoogle Scholar
- Couly GF, Coltey PM, Le Douarin NM: The triple origin of skull in higher vertebrates: a study in quail-chick chimeras. Development 1993, 117:409–429.PubMedGoogle Scholar
- Chai Y, Jiang XB, Ito Y, Bringas P, Han J, Rowitch DH, Soriano P, McMahon AP, Sucov HM: Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis. Development 2000, 127:1671–1679.PubMedGoogle Scholar
- O’Gorman S: Second branchial arch lineages of the middle ear of wild-type and Hoxa2 mutant mice. Dev Dyn 2005, 234:124–131.PubMedView ArticleGoogle Scholar
- Westoll TS: Ancestry of the tetrapods. Nature 1938, 141:127–128.View ArticleGoogle Scholar
- Schultze HP, Arsenault M: The panderichthyid fish Elpistostege : a close relative of tetrapods? Palaeontology 1985, 28:293–309.Google Scholar
- Gegenbaur C: Elements of Comparative Anatomy. London: MacMillan & Co.; 1878.Google Scholar
- Gaupp E: Die Entwicklung des Kopfskelettes. In Handbuch der Vergleichenden und Experimentellen Entwicklungslehre der Wirbeltiere, Bd 3(2). Edited by Hertwig O. Jena: Gustav Fischer; 1906:573–874.Google Scholar
- Wiedersheim R: Vergleichende Anatomie der Wirbeltiere: Für Studierende bearbeitet. Jena: Verlag von Gustav Fischer; 1909.Google Scholar
- Goodrich ES: Studies on the Structure and Development of Vertebrates. London: Macmillan; 1930.View ArticleGoogle Scholar
- Portmann A: Einführung in die vergleichende Morphologie der Wirbeltiere. Basel: Schwabe & Co; 1969.Google Scholar
- Jarvik E: Basic Structure and Evolution of Vertebrates, Volume 2. New York: Academic Press; 1980.Google Scholar
- Moore WJ: The Mammalian Skull. London: Cambridge University Press; 1981.Google Scholar
- Hanken J, Hall BK: The Skull, Volume 1–3. Chicago: University of Chicago Press; 1993.Google Scholar
- Hall BK: The Neural Crest in Development and Evolution. New York: Springer Verlag; 1999.View ArticleGoogle Scholar
- Wada N, Nohno T, Kuratani S: Dual origins of the prechordal cranium in the chicken embryo. Dev Biol 2011, 356:529–540.PubMedView ArticleGoogle Scholar
- Kuratani S, Adachi N, Wada N, Oisi Y, Sugahara F: Developmental and evolutionary significance of the mandibular arch and prechordal/premandibular cranium in vertebrates: revising the heterotopy scenario of gnathostome jaw evolution. J Anat 2013, 222:41–55.PubMed CentralPubMedView ArticleGoogle Scholar
- Kuratani S, Matsuo I, Aizawa S: Developmental patterning and evolution of the mammalian viscerocranium: Genetic insights into comparative morphology. Dev Dyn 1997, 209:139–155.PubMedView ArticleGoogle Scholar
- Kuratani S: Evolution of the vertebrate jaw from developmental perspectives. Evol Dev 2012, 14:76–92.PubMedView ArticleGoogle Scholar
- Oisi Y, Ota KG, Kuraku S, Fujimoto S, Kuratani S: Craniofacial development of hagfishes and the evolution of vertebrates. Nature 2013, 493:175–180.PubMedView ArticleGoogle Scholar
- Oisi Y, Ota KG, Fujimoto S, Kuratani S: Development of the chondrocranium in hagfishes, with special reference to the early evolution of vertebrates. Zool Sci 2013, 30:944–961.PubMedView ArticleGoogle Scholar
- Dupret V, Sanchez S, Goujet D, Tafforeau P, Ahlberg PE: A primitive placoderm sheds light on the origin of the jawed vertebrate face. Nature 2014, 507:500–503.PubMedView ArticleGoogle Scholar
- Fürbringer M: Über die spino-occipitalen Nerven der Selachier und Holocephalen und ihre vergleichende Morphologie. Festschr für Carl Gegenbaur 1897, 3:349–788.Google Scholar
- Kessel M, Balling R, Gruss P: Variations of cervical vertebrae after expression of a Hox-1.1 transgene in mice. Cell 1990, 61:301–308.PubMedView ArticleGoogle Scholar
- Lufkin T, Mark M, Hart CP, Dolle P, Lemeur M, Chambon P: Homeotic transformation of the occipital bones of the skull by ectopic expression of a homeobox gene. Nature 1992, 359:835–841.PubMedView ArticleGoogle Scholar
- Kessel M: Respecification of vertebral identities by retinoic acid. Development 1992, 115:487–501.PubMedGoogle Scholar
- Oken L: Über die Bedeutung der Schädelknochen. Bamberg: Göbhardt; 1807.Google Scholar
- Owen RC: On the Archetype and Homologies of the Vertebrate Skeleton. London: J. Van Voorst; 1848.Google Scholar
- Huxley TH: The Croonian Lecture: on the theory of the vertebrate skull. Proc Zool Soc Lond 1858, 9:381–457.Google Scholar
- Olsson L, Hanken J: Cranial neural crest migration and chondrogenic fate in the oriental fire-bellied toad Bombina orientalis : defining the ancestral pattern of head development in anuran amphibians. J Morphol 1996, 229:105–120.View ArticleGoogle Scholar
- Gross JB, Hanken J: Segmentation of the vertebrate skull: neural-crest derivation of adult cartilages in the clawed frog, Xenopus laevis . Intg Comp Biol 2008, 48:681–696.View ArticleGoogle Scholar
- Hanken J, Gross JB: Evolution of cranial development and the role of neural crest: insights from amphibians. J Anat 2005, 207:437–446.PubMed CentralPubMedView ArticleGoogle Scholar
- Jollie M: Segment theory and the homologizing of cranial bones. Am Nat 1981, 118:785–802.View ArticleGoogle Scholar
- Thomson KS: Segmentation, the adult skull, and the problem of homology. In The Skull, Vol 2. Edited by Hanken J, Hall BK. Chicago: University of Chicago Press; 1993:36–68.Google Scholar
- Heintz A: The structure of Dinichthys : a contribution to our knowledge of the arthrodira. In Bashford Dean Memorial Volume: Archaic Fishes. Edited by Gudger EW. New York: American Museum of Natural History; 1932:111–241.Google Scholar
- Wada H, Ghysen A, Satou C, Higashijima S, Kawakami K, Hamaguchi S, Sakaizumi M: Dermal morphogenesis controls lateral line patterning during postembryonic development of teleost fish. Dev Biol 2010, 340:583–594.PubMedView ArticleGoogle Scholar
- Schlosser G: Making senses: development of vertebrate cranial placodes. Int Rev Cell Mol Biol 2010, 283:129–234.PubMedView ArticleGoogle Scholar
- Noden DM: The role of the neural crest in patterning of avian cranial skeletal, connective, and muscle tissues. Dev Biol 1983, 96:144–165.PubMedView ArticleGoogle Scholar
- Evans DJR, Noden DM: Spatial relations between avian craniofacial neural crest and paraxial mesoderm cells. Dev Dyn 2006, 235:1310–1325.PubMedView ArticleGoogle Scholar
- Le Lièvre CS: Participation of neural crest-derived cells in the genesis of the skull in birds. J Embryol Exp Morph 1978, 47:17–37.PubMedGoogle Scholar
- Le Douarin NM, Dupin E: The neural crest in vertebrate evolution. Curr Opin Genet Dev 2012, 22:381–389.PubMedView ArticleGoogle Scholar
- McGonnell IM, Graham A: Trunk neural crest has skeletogenic potential. Curr Biol 2002, 12:767–771.PubMedView ArticleGoogle Scholar
- Nakamura H, Ayer-Le Lièvre CS: Mesectodermal capabilities of the trunk neural crest of birds. J Embryol Exp Morph 1982, 70:1–18.PubMedGoogle Scholar
- Cebra-Thomas JA, Terrell A, Branyan K, Shah S, Rice R, Gyi L, Yin M, Hu YS, Mangat G, Simonet J, Betters E, Gilbert SF: Late-emigrating trunk neural crest cells in turtle embryos generate an osteogenic ectomesenchyme in the plastron. Dev Dyn 2013, 242:1223–1235.PubMedView ArticleGoogle Scholar
- Mongera A, Nüsslein-Volhard C: Scales of fish arise from mesoderm. Curr Biol 2013, 23:R338–R339.PubMedView ArticleGoogle Scholar
- Cebra-Thomas JA, Betters E, Yin M, Plafkin C, Mcdow K, Gilbert SF: Evidence that a late-emerging population of trunk neural crest cells forms the plastron bones in the turtle Trachemys scripta . Evol Dev 2007, 9:267–277.PubMedView ArticleGoogle Scholar
- Reif WE: Evolution of dermal skeleton and dentition in vertebrates: the odontode regulation theory. Evol Biol 1982, 15:287–368.View ArticleGoogle Scholar
- Noden DM: Control of avian cephalic neural crest cytodifferentiation. I. skeletal and connective tissues. Dev Biol 1978, 67:296–312.PubMedView ArticleGoogle Scholar
- Koyabu D, Maier W, Sánchez-Villagra MR: Paleontological and developmental evidence resolve the homology and dual embryonic origin of a mammalian skull bone, the interparietal. Proc Natl Acad Sci U S A 2012, 109:14075–14080.PubMed CentralPubMedView ArticleGoogle Scholar
- Wagner G: Untersuchungen an Bombinator-Triton -Chimaeren. Roux’s Arch Ent mech Org 1959, 151:136–158.View ArticleGoogle Scholar
- Rijli FM, Mark M, Lakkaraju S, Dierich A, Dollé P, Chambon P: A homeotic transformation is generated in the rostral branchial region of the head by disruption of Hoxa-2 , which acts as a selector gene. Cell 1993, 75:1333–1349.PubMedView ArticleGoogle Scholar
- Couly G, Grapin-Botton A, Coltey P, Ruhin B, Le Douarin NM: Determination of the identity of the derivatives of the cephalic neural crest: incompatibility between Hox gene expression and lower jaw development. Development 1998, 125:3445–3459.PubMedGoogle Scholar
- Schneider RA, Helms JA: The cellular and molecular origins of beak morphology. Science 2003, 299:565–568.PubMedView ArticleGoogle Scholar
- Couly G, Creuzet S, Bennaceur S, Vincent C, Le Douarin NM: Interactions between Hox-negative cephalic neural crest cells and the foregut endoderm in patterning the facial skeleton in the vertebrate head. Development 2002, 129:1061–1073.PubMedGoogle Scholar
- Westoll TS: On the evolution of the Dipnoi. In Genetics, Paleontology and Evolution. Edited by Jepson GL, Simpson GG, Mayr E. Princeton: Princeton University Press; 1949:112–184.Google Scholar
- White EI: A little on lungfishes. Proc Linn Soc Lond 1966, 177:1–10.View ArticleGoogle Scholar
- Thomson KS, Campbell KSW: The structure and relationships of the primitive Devonian lungfish: Dipnorhynchus sussmilchi (Etheridge). Bull Peabody Mus Nat Hist Yale 1971, 38:1–109.Google Scholar
- Gross JB, Hanken J: Cranial neural crest contributes to the bony skull vault in adult Xenopus laevis : insights from cell labeling studies. J Exp Zool B (MDE) 2005, 304B:169–176.View ArticleGoogle Scholar
- Schneider RA: Neural crest can form cartilages normally derived from mesoderm during development of the avian head skeleton. Dev Biol 1999, 208:441–455.PubMedView ArticleGoogle Scholar
- Showing J: Mise en évidence du rôle inducteur de l’encephale dans l’ostéogenèse du crâne embryonaire du poulet. J Embryol Exp Morph 1993, 75:165–188.Google Scholar
- McGonnell IM, McKay IJ, Graham A: A population of caudally migrating cranial neural crest cells: functional and evolutionary implications. Dev Biol 2001, 236:354–363.PubMedView ArticleGoogle Scholar
- Epperlein HH, Khattak S, Knapp D, Tanaka EM, Malashichev YB: Neural crest does not contribute to the neck and shoulder in the Axolotl ( Ambystoma mexicanum ). PLoS ONE 2012, 7:e52244.PubMed CentralPubMedView ArticleGoogle Scholar
- Wagner GP, Gauthier JA: 1,2,3 = 2,3,4: A solution to the problem of the homology of the digits in the avian hand. Proc Natl Acad Sci U S A 1999, 96:5111–5116.PubMed CentralPubMedView ArticleGoogle Scholar
- Tamura K, Nomura N, Seki R, Yonei-Tamura S, Yokoyama H: Embryological evidence identifies wing digits in birds as digits 1, 2, and 3. Science 2011, 331:753–757.PubMedView ArticleGoogle Scholar
- Xu X, Mackem S: Tracing the evolution of avian wing digits. Curr Biol 2013, 23:R538–R544.PubMedView ArticleGoogle Scholar
- Burke AC, Nelson CE, Morgan BA, Tabin C: Hox genes and the evolution of vertebrate axial morphology. Development 1995, 121:333–346.PubMedGoogle Scholar
- Narita Y, Kuratani S: Evolution of the vertebral formulae in mammals: A perspective on developmental constraints. J Exp Zool B (MDE) 2005, 304B:91–106.View ArticleGoogle Scholar
- Carroll SB, Greiner JK, Weatherbee SD: From DNA to Diversity: Molocular Genetics and the Evolution of Animal Design. 2nd edition. Malden: Wiley; 2005.Google Scholar
- Arendt D: The evolution of cell types in animals: emerging principles from molecular studies. Nat Rev Genet 2008, 9:868–882.PubMedView ArticleGoogle Scholar
- Ahlberg PE, Koentges G: Homologies and cell populations: a response to Sánchez-Villagra and Maier. Evol Dev 2006, 8:116–118.PubMedView ArticleGoogle Scholar
- Sánchez-Villagra MR, Maier W: Homologies of the mammalian shoulder girdle: a response to Matsuoka et al. (2005). Evol Dev 2006, 8:113–115.PubMedView ArticleGoogle Scholar
- Scotland RW: Deep homology: A view from systematics. Bioessays 2010, 32:438–449.PubMedView ArticleGoogle Scholar
- Roth VL: The biological basis of homology. In Ontogeny and Systematics. Edited by Humphries CJ. New York: Columbia University Press; 1988:1–26.Google Scholar
- True JR, Haag ES: Developmental system drift and flexibility in evolutionary trajectories. Evol Dev 2001, 3:109–119.PubMedView ArticleGoogle Scholar
- Kotthaus A: Die Entwicklung des Primordial-Craniums von Xenopus laevis bis zur Metamorphose. Zeit wiss Zool 1933, 144:510–572.Google Scholar
- De Sá RO, Swart CC: Development of the suprarostral plate of pipoid frogs. J Morphol 1999, 240:143–153.PubMedView ArticleGoogle Scholar
- Eames BF, Helms JA: Conserved molecular program regulating cranial and appendicular skeletogenesis. Dev Dyn 2004, 231:4–13.PubMedView ArticleGoogle Scholar
- Gould SJ, Lewontin RC: The spandrels of San Marco and the Panglossian paradigm: a critique of the adaptationist programme. Proc Roy Soc B 1979, 205:581–598.View ArticleGoogle Scholar
- Olson ME: The developmental renaissance in adaptationism. Trends Ecol Evol 2012, 27:278–287.PubMedView ArticleGoogle Scholar
- Duboule D: Temporal colinearity and the phylotypic progression: a basis for the stability of a vertebrate bauplan and the evolution of morphologies through heterochrony. Development 1994, ᅟ:Supplement: 135–142.Google Scholar
- Irie N, Kuratani S: Comparative transcriptome analysis reveals vertebrate phylotypic period during organogenesis. Nat Commun 2011, 2:248.PubMed CentralPubMedView ArticleGoogle Scholar
- Wang Z, Pascual-Anaya J, Zadissa A, Li WQ, Niimura Y, Huang ZY, Li CY, White S, Xiong ZQ, Fang DM, Wang B, Ming Y, Chen Y, Zheng Y, Kuraku S, Pignatelli M, Herrero J, Beal K, Nozawa M, Li QY, Wang J, Zhang HY, Yu LL, Shigenobu S, Wang JY, Liu JN, Flicek P, Searle S, Wang J, Kuratani S et al: The draft genomes of soft-shell turtle and green sea turtle yield insights into the development and evolution of the turtle-specific body plan. Nat Genet 2013, 45:701–706.PubMed CentralPubMedView ArticleGoogle Scholar
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